# US 10,767,206 B2 · Nitrogen source feeding fermentation process for producing gellan gum

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- Number: US 10,767,206 B2
- Document ID: US10767206B2
- Status: Granted
- Kind: B2
- Application number: 16/012,653
- Filed: 2018-06-19
- Published: 2019-01-03
- Granted: 2020-09-08
- Earliest claimed: 2017-06-29
- Assignees: Dsm IP Assets B.V.
- Applicants: Dsm IP Assets B.V.
- Inventors: Jie Zhao; Zhihui Gao; Wouter Adrianus Van Winden; Rogier Meulenberg
- Web page: https://patentgrep.com/patent/US10767206B2

## CPC

- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum.

## Claims

### Claim 1

A fermentation process for producing a gellan gum, comprising the following steps:
1) inoculating a culture medium with a strain capable of producing a gellan gum to form a broth; and
2) initiating a fermentation, and feeding a nitrogen source into the broth during the fermentation;
wherein the strain capable of producing the gellan gum is selected from a group consisting of Sphingomonas paucimobilis, Sphingomonas Azotofigens, and Sphingomonas elodea:
wherein the nitrogen source is fed into the broth during the fermentation after inoculation with the strain according to FIG. 1 feeding curve diagram, which falls into the shaded area of FIG. 1;
wherein a total amount of the nitrogen source fed into the broth is 0.01 mol/L to 0.1 mol/L calculated as the nitrogen element in the broth in relative to the total volume of the broth.

### Claim 2 (depends on 1)

The fermentation process of claim 1, wherein the nitrogen source is selected from the group consisting of the following one or more of nitrogen-containing materials: glutamic acid, monosodium glutamate, aqueous ammonia, (NH4)2SO4, NaNO3, KNO3, NH4NO3, NH4CI, yeast extract, (NH4)3PO4, (NH4)2HPO4, NH4H2PO4, NH4OH, and soybean powder.

### Claim 3 (depends on 1)

The fermentation process of claim 1, wherein the total amount of the nitrogen source fed into the broth is 0.015 mol/L to 0.08 mol/L, or 0.02 mol/L to 0.04 mol/L, which is calculated as the nitrogen element in the broth in relative to the total volume of the broth.

### Claim 4 (depends on 1)

The fermentation process of claim 1, wherein the nitrogen source is fed in any one of below manners into the broth during the fermentation after inoculation with a strain:
1) the nitrogen source is intermittently fed into the broth in a pulsed feeding mode at certain intervals;
2) the nitrogen source is continuously fed into the broth with a constant feeding speed and the feeding speed is kept constant during the fermentation;
3) the nitrogen source is continuously fed into the broth with a constant feeding speed, and the feeding speed is kept higher in an early stage and lower in a late stage, or the feeding speed is kept lower in an early stage and higher in a late stage, during the fermentation;
4) the nitrogen source is continuously fed into the broth with a constant feeding speed, and it is fed in an early stage while not fed in a late stage, or it is not fed in an early stage while fed in a late stage, during the fermentation;
5) the nitrogen source is continuously fed into the broth with an exponentially changing feeding speed; or
6) the nitrogen source is continuously fed into the broth with a linearly changing feeding speed.

### Claim 5 (depends on 1)

The fermentation process of claim 1, further comprising the following step:
stopping the fermentation, and isolating and purifying the gellan gum produced during the fermentation.

### Claim 6 (depends on 1)

The fermentation process of claim 1, wherein the culture medium contains no nitrogen source.