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US 10,767,205 B2Granted
Bacterium for the production of an exopolysaccharide comprising N-acetylglucosamine and D-glucose
AssigneeDePuy Synthes Products, Inc.
InventorDouglas D. Buechter
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Abstract
The present disclosure is directed to microorganisms that are genetically modified to express at least one exogenous enzyme involved in N-acetylglucosamine uptake and metabolism. Methods for the production of an exopolysaccharide are also disclosed. The exopolysaccharide comprises N-acetylglucosamine and D-glucose and may be used as a bioresorbable implant for soft tissue repair, replacement, or augmentation.
Claims2
A bacterium for the production of an exopolysaccharide comprising N-acetylglucosamine and D-glucose, wherein:
- the bacterium belongs to a genus comprising Komagataeibacter, Agrobacterium, Aerobacter, Achromobacter, Azotobacter, Rhizobium, Sarcina, or Salmonella and the bacterium expresses
- a. an Escherichia coli N-acetylglucosamine phosphoenolpyruvate-dependent sugar phosphotransferase system permease;
- and
- b. an Escherichia coli N-acetylglucosamine kinase, a Mus musculus phosphoacetyl-glucosamine mutase, and an Escherichia coli bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase, and
- the bacterium produces an exopolysaccharide that comprises N-acetylglucosamine and D-glucose.
2 more elements
Dependencies are inferred from the claim text.