Arylalkylamine N-acetyltransferase and uses thereof
AssigneeDanmarks Tekniske Universitet
InventorHao Luo
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Abstract
Described herein are variants of arylalkylamine N-acetyltransferase (AANAT) as well as vectors and recombinant microbial host cells expressing AANATs and variant AANATs and their use in producing N-acetylserotonin, melatonin and related compounds. Preferred AANAT variants provide for an increased yield of N-acetylserotonin.
Claims20
A catalytically active variant of arylalkylamine N-acetyltransferase (AANAT) from Streptomyces griseofuscus (SEQ ID NO:1), the variant AANAT having at least 85% sequence identity to SEQ ID NO:1, comprising a mutation in residue D63 and providing for an increased yield in vivo of N-acetylserotonin from serotonin relative to native enzyme.
A catalytically active variant of AANAT from Streptomyces griseofuscus (SEQ ID NO:1), wherein the variant AANAT comprises SEQ ID NO:1 and has a D63G amino acid substitution.
A recombinant host cell which is derived from an E. coli or S. cerevisiae cell, comprising a chromosomally integrated gene encoding an AANAT having at least 85% identity to SEQ ID NO:
- 1 from Streptomyces griseofuscus or a catalytically active fragment or variant thereof, wherein said AANAT or catalytically active fragment thereof comprises a D63 amino acid substitution.
Dependencies are inferred from the claim text.