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US 10,767,201 B2Granted
CYP76AD1-beta clade polynucleotides, polypeptides, and uses thereof
AssigneeYeda Research and Development Co. Ltd.
InventorsAsaph Aharoni, Guy Polturak
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AssigneeYeda Research and Development Co. Ltd.
InventorsAsaph Aharoni, Guy Polturak
Estimating the term…
The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4.5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.
A recombinant polynucleotide comprising a nucleic acid sequence encoding (i) a cytochrome P450 enzyme CYP76AD6 that converts tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), wherein the nucleic acid sequence encoding the CYP76AD6 enzyme is set forth in SEQ ID NO:31, or (ii) a cytochrome P450 enzyme CYP76AD15 that converts tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), wherein the nucleic acid sequence encoding the CYP76AD15 enzyme is set forth in SEQ ID NO:35, wherein said nucleic acid encoding the CYP76AD6 enzyme or the CYP76AD15 enzyme is under the control of a promoter, and wherein said promoter is a constitutive promoter, an inducible promoter, a fruit-specific promoter, or a flower-specific promoter.
Dependencies are inferred from the claim text.