# US 10,767,192 B2 · Baculovirus system for the expression of a gene therapy vector > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,767,192 B2 - Document ID: US10767192B2 - Status: Granted - Kind: B2 - Application number: 16/029,870 - Filed: 2018-07-09 - Published: 2018-10-25 - Granted: 2020-09-08 - Earliest claimed: 2011-07-27 - Assignees: Genethon - Applicants: Genethon - Inventors: Lionel Galibert; Otto-Wilhelm Merten; Aurelien Jacob - Web page: https://patentgrep.com/patent/US10767192B2 ## CPC - C12N 15/86: Viral vectors - C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) - C12N 2710/14044: Chimeric viral vector comprising heterologous viral elements for production of another viral vector - C12N 2750/14043: viral genome or elements thereof as genetic vector - C12N 2750/14141: Use of virus, viral particle or viral elements as a vector - C12N 2750/14151: Methods of production or purification of viral material - C12N 2800/50: Vectors for producing vectors - C12N 2830/20: transcription of more than one cistron - C12N 2999/007: Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The invention relates to a recombinant baculoviral genome useful for the production of viral vectors for gene therapy, allowing said production from a single infection. ## Claims ### Claim 1 A recombinant baculovirus genome comprising: (i) one or more expression cassettes comprising adeno-associated virus (AAV) rep and cap genes under the control of a promoter suitable for expression of said genes in an insect host cell, and (ii) a recombinant genome of an AAV vector, wherein said one or more expression cassettes and said recombinant genome of an AAV vector are each inserted into a locus selected from the egt locus and the polyhedrin locus. ### Claim 2 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, said rep and cap genes being contained in a single expression cassette. ### Claim 3 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein said one or more expression cassettes and said recombinant genome of an AAV vector are each inserted into a different locus. ### Claim 4 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein said one or more expression cassettes is(are) inserted into the egt locus. ### Claim 5 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein said one or more expression cassettes is(are) inserted into the polyhedrin locus. ### Claim 6 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein said recombinant genome of an AAV vector is inserted into the egt locus. ### Claim 7 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein said recombinant genome of an AAV vector is inserted into the polyhedrin locus. ### Claim 8 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein: said one or more expression cassettes is(are) inserted into the polyhedrin locus; and said recombinant genome of an AAV vector is inserted into the egt locus. ### Claim 9 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein: said one or more expression cassettes is(are) inserted into the egt locus; and said recombinant genome of an AAV vector is inserted into the polyhedrin locus. ### Claim 10 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein the chitinase, cathepsin and p10 genes are deleted. ### Claim 11 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, said baculovirus genome being derived from AcMNPV. ### Claim 12 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, wherein the AAV recombinant genome comprises a heterologous gene encoding a protein, an interfering RNA or an antisense RNA, which is therapeutic. ### Claim 13 (depends on 1) The recombinant baculovirus genome as claimed in claim 1, said recombinant baculovirus genome being a bacmid. ### Claim 14 (depends on 1) A recombinant baculovirus containing a recombinant baculovirus genome as claimed in claim 1. ### Claim 15 (depends on 1) A eukaryotic or prokaryotic cell containing the recombinant baculovirus genome as claimed in claim 1. ### Claim 16 (depends on 15) The cell as claimed in claim 15, said cell being an insect cell derived from the Spodoptera frugiperda or Trichoplusia ni lines. ### Claim 17 (depends on 16) The cell as claimed in claim 16, said cell being selected from the group consisting of Sf21, Sf9, TN 5B1-4 and High Five cells. ### Claim 18 (depends on 14) A method for producing a recombinant virus comprising culturing a cell with one or more recombinant baculovirus as claimed in claim 14 under conditions which allow the infection of the cell with the baculovirus and the production of said recombinant virus. ### Claim 19 (depends on 14) A eukaryotic cell infected with the recombinant baculovirus of claim 14. ### Claim 20 (depends on 19) The cell as claimed in claim 19, said cell being an insect cell derived from the Spodoptera frugiperda or Trichoplusia ni lines. ### Claim 21 (depends on 20) The cell as claimed in claim 20, said cell being selected from the group consisting of SF21, SF9, TN 5B1-4 and High Five cells.