# US 10,767,158 B2 · Bioprotection using lactobacillus paracasei strains > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,767,158 B2 - Document ID: US10767158B2 - Status: Granted - Kind: B2 - Application number: 16/030,615 - Filed: 2018-07-09 - Published: 2019-01-10 - Granted: 2020-09-08 - Earliest claimed: 2012-04-09 - Assignees: Chr. Hansen A/S - Applicants: Chr. Hansen A/S - Inventors: Tina Hornbaek; Maike Lisberg; Silja Kej Diemer - Web page: https://patentgrep.com/patent/US10767158B2 ## CPC - C12N 1/20: Bacteria; Culture media therefor - A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom - A23B 2/783: Microorganisms; Enzymes - A23C 9/1234: {characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.} - A23C 13/16: containing, or treated with, microorganisms, enzymes, or antibiotics; Sour cream - A23C 19/0323: {using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general (using moulds A23C19/0682)} - A23C 19/076: Soft unripened cheese, e.g. cottage or cream cheese - A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics - A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis - A61P 31/10: Antimycotics - C12N 1/205: Bacterial isolates - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - A23V 2400/165: Paracasei - A23V 2400/175: Rhamnosus - C12R 2001/225: Lactobacillus - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A23B: PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES - A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00) - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention is related to the field of bioprotection, in particular to the strain of Lactobacillus paracasei CHCC14676 with accession no. DSM25612. Furthermore, the present invention concerns an antifungal composition comprising the strain, an antifungal composition comprising the strain and at least one strain of Lactobacillus rhamnosus, food, feed and pharmaceutical products comprising such an antifungal composition, a method of manufacturing such food, feed and pharmaceutical products, a method for reducing the content of yeasts and molds of such food, feed and pharmaceutical products and uses of the antifungal composition. ## Claims ### Claim 1 An antifungal composition comprising at least one Lactobacillus paracasei strain selected from Lactobacillus paracasei strain CHCC14676 (DSM25612) and mutant strains derived therefrom, wherein the mutant strains have substantially the same or improved antifungal properties as CHCC14676 (DSM25612), wherein the Lactobacillus paracasei strain is capable of inhibiting or preventing the growth of Mucor spp, and wherein the composition comprises an amount of the at least one Lactobacillus paracasei strain effective to inhibit growth of yeasts and molds in the composition and further comprises an effective amount of an added cryoprotectant for the at least one Lactobacillus paracasei strain. ### Claim 2 (depends on 1) The composition according to claim 1, wherein the composition comprises at least 104 colony forming units of the Lactobacillus paracasei strains per gram of the composition. ### Claim 3 (depends on 1) The composition according to claim 1, wherein the cryoprotectant is selected from one or more of mannitol, sorbitol, sodium tripolyphosphate, xylitol, glycerol, raffinose, maltodextrin, erythritol, threitol, trehalose, glucose and fructose. ### Claim 4 (depends on 1) The composition according to claim 1, further comprising an additive selected from one or more of yeast extracts, sugars, vitamins, carbohydrates, flavors, minerals, and enzymes. ### Claim 5 (depends on 1) The composition according to claim 1, further comprising at least one strain of Lactobacillus rhamnosus. ### Claim 6 (depends on 1) The composition according to claim 1, wherein the composition is in a liquid, frozen, dried, or freeze-dried form. ### Claim 7 A food, feed or pharmaceutical product, comprising at least one Lactobacillus paracasei strain selected from Lactobacillus paracasei strain CHCC14676 (DSM25612) and mutant strains derived therefrom and an effective amount of an added cryoprotectant for the at least one Lactobacillus paracasei strain, wherein the mutant strains have substantially the same or improved antifungal properties as CHCC14676 (DSM25612), the Lactobacillus paracasei strain is capable of inhibiting or preventing the growth of Mucor spp., and the food, feed or pharmaceutical product comprises an amount of the at least one Lactobacillus paracasei strain effective to inhibit growth of yeasts and molds in the food, feed or pharmaceutical product. ### Claim 8 (depends on 7) The food, feed or pharmaceutical product according to claim 7, comprising an amount of the at least one Lactobacillus paracasei strain selected from at least 1×106 cfu/g of the food, feed or pharmaceutical product, at least 1×106 cfu/ml of the food, feed or pharmaceutical product, or at least 1×105 cfu/cm2 of the surface of the food, feed or pharmaceutical product. ### Claim 9 (depends on 7) The food, feed or pharmaceutical product according to claim 7, further comprising at least one strain of Lactobacillus rhamnosus. ### Claim 10 A food, feed or pharmaceutical product made by a process comprising adding at least one Lactobacillus paracasei strain selected from Lactobacillus paracasei strain CHCC14676 (DSM25612) and mutant strains derived therefrom and an effective amount of an added cryoprotectant for the at least one Lactobacillus paracasei strain to a food, feed or pharmaceutical product during manufacture in an amount effective to inhibit growth of yeasts and molds in the food, feed or pharmaceutical product, wherein the mutant strains have substantially the same or improved antifungal properties as CHCC14676 (DSM25612), and wherein the Lactobacillus paracasei strain is capable of inhibiting or preventing the growth of Mucor spp. ### Claim 11 (depends on 10) The food, feed or pharmaceutical product according to claim 10, comprising an amount of the at least one Lactobacillus paracasei strain selected from at least 1×106 cfu/g of the food, feed or pharmaceutical product, at least 1×106 cfu/ml of the food, feed or pharmaceutical product, or at least 1×105 cfu/cm2 of the surface of the food, feed or pharmaceutical product. ### Claim 12 (depends on 10) The food, feed or pharmaceutical product according to claim 10, further comprising at least one strain of Lactobacillus rhamnosus.