# US 10,766,937 B2 · Compositions and methods of treating cancer > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,766,937 B2 - Document ID: US10766937B2 - Status: Granted - Kind: B2 - Application number: 14/343,022 - Filed: 2012-09-06 - Published: 2015-02-12 - Granted: 2020-09-08 - Earliest claimed: 2011-09-06 - Assignees: Dana-Farber Cancer Institute, Inc. - Applicants: Yoshihiro Nakatani - Inventors: Yoshihiro Nakatani - Web page: https://patentgrep.com/patent/US10766937B2 ## CPC - C07K 14/4747: {Apoptosis related proteins} - C07K 14/4702: {Regulators; Modulating activity} - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract The present invention provides compositions and methods of treating various disorders associated with aberrant cell growth. ## Claims ### Claim 1 A p600 deoxyribonucleic acid (DNA) fragment consisting of a DNA sequence selected from the group consisting of SEQ ID NO: 7, 9, 11, 13, 15, 17, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 wherein the p600 DNA fragment encodes a polypeptide that when expressed in a cell induces cell death. ### Claim 2 (depends on 1) The p600 DNA fragment of claim 1, wherein the p600 DNA fragment is SEQ ID NO: 21. ### Claim 3 (depends on 1) The DNA fragment of claim 1, wherein the DNA fragment is in a vector. ### Claim 4 (depends on 1) A composition comprising the p600 DNA fragment of claim 1 and a pharmaceutically acceptable carrier. ### Claim 5 A nanoparticle or nanosphere comprising a p600 DNA fragment consisting of a DNA sequence selected from the group consisting of SEQ ID NO: 3, 5, 7, 9, 11, 13 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79, wherein the DNA fragment encodes a polypeptide that when expressed in a cell induces cell death. ### Claim 6 A p600 DNA fragment selected from the group consisting of a p600 DNA sequence comprising SEQ ID NO: 7 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 9 that is less than 2,000 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 11 that is less than 2,900 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 13 that is less than 2,200 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 15 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 17 that is less than 1,900 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 21 that is less than 1,900 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 23 that is less than 1,700 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 25 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 27 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 29 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 31 that is less than 2,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 33 that is less than 1,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 35 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 37 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 39 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 41 that is less than 2,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 43 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 45 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 47 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 49 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 51 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 53 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 55 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 57 that is less than 900 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 59 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 61 that is less than 1,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 63 that is less than 1,100 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 65 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 67 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 69 that is less than 2,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 71 that is less than 2,400 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 73 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 75 that is less than 1,600 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 77 that is less than 1,700 nucleotides in length, and a p600 DNA sequence comprising of SEQ ID NO: 79 that is less than 1,800 nucleotides in length, wherein the p600 DNA fragment encodes a polypeptide that when expressed in a cell induces cell death. ### Claim 7 (depends on 6) The p600 DNA fragment of claim 6, wherein the DNA fragment is in a vector. ### Claim 8 (depends on 6) A composition comprising the p600 DNA fragment of claim 6 and a pharmaceutically acceptable carrier. ### Claim 9 A nanoparticle or nanosphere comprising a p600 DNA fragment selected from the group consisting of a p600 DNA sequence comprising SEQ ID NO: 3 that is less than 2,600 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 5 that is less than 2,200 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 7 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 9 that is less than 2,000 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 11 that is less than 2,900 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 13 that is less than 2,200 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 15 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 17 that is less than 1,900 nucleotides in length, a p600 DNA sequence comprising SEQ ID NO: 19 that is less than 2,200 nucleotides in length, and a p600 DNA sequence comprising SEQ ID NO: 21 that is less than 1,900 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 23 that is less than 1,700 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 25 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 27 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 29 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 31 that is less than 2,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 33 that is less than 1,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 35 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 37 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 39 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 41 that is less than 2,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 43 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 45 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 47 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 49 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 51 that is less than 1,200 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 53 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 55 that is less than 1,000 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 57 that is less than 900 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 59 that is less than 800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 61 that is less than 1,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 63 that is less than 1,100 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 65 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 67 that is less than 1,500 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 69 that is less than 2,300 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 71 that is less than 2,400 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 73 that is less than 1,800 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 75 that is less than 1,600 nucleotides in length, a p600 DNA sequence comprising of SEQ ID NO: 77 that is less than 1,700 nucleotides in length, and a p600 DNA sequence comprising of SEQ ID NO: 79 that is less than 1,800 nucleotides in length, wherein the p600 DNA fragment encodes a polypeptide that when expressed in a cell induces cell death.