# US 10,766,924 B2 · Affinity support and method for isolating immunoglobulin > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,766,924 B2 - Document ID: US10766924B2 - Status: Granted - Kind: B2 - Application number: 15/745,855 - Filed: 2016-07-27 - Published: 2018-08-09 - Granted: 2020-09-08 - Earliest claimed: 2015-07-28 - Assignees: Jsr Corporation; Jsr Life Sciences Corporation - Applicants: Jsr Corporation; Jsr Life Sciences Corporation - Inventors: Takashi Ichii; Satoshi Nakamura; Jun-ichi Yasuoka; Kaori Itaya; Tomonori Shiotani - Web page: https://patentgrep.com/patent/US10766924B2 ## CPC - C07K 1/22: Affinity chromatography or related techniques based upon selective absorption processes - B01J 20/289: bonded via a spacer - B01J 20/3204: {Inorganic carriers, supports or substrates} - B01J 20/321: {consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds} - B01J 20/3219: {involving a particular spacer or linking group, e.g. for attaching an active group} - B01J 20/3248: {the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such} - B01J 20/3255: {comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures} - G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 - G01N 2030/8831: {involving peptides or proteins} - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract To provide an affinity support in which a binding property of a ligand to a target substance is improved. The affinity support contains a solid phase support and a protein ligand, wherein the protein ligand is represented by formula (1): R—R1 (1) wherein R represents a linker binding to the solid phase support, which contains a polyproline, and R1 represents a protein showing an affinity to immunoglobulin, and the R is bound to a C terminal or an N terminal of an amino acid sequence in R1. ## Claims ### Claim 1 An affinity support, comprising: a solid phase support and a protein ligand, wherein the protein ligand has the following formula(1): R—R1 (1), wherein R is a linker binding to the solid phase support, which comprises a polyproline comprising from 12 to 24 proline residues, and R1 is a protein having an affinity to an immunoglobulin, and R is bound to a C terminal or an N terminal of an amino acid sequence of the protein R1. ### Claim 2 (depends on 1) The affinity support according to claim 1, wherein the linker has a length of from 3.6 nm to 7.3 nm. ### Claim 3 (depends on 1) The affinity support according to claim 1, wherein the linker comprises an amino acid residue having an amino group or a thiol group at a terminal binding to the solid phase support. ### Claim 4 (depends on 1) The affinity support according to claim 1, wherein the protein having the affinity to an immunoglobulin comprises an immunoglobulin-binding domain derived from an Fc-binding protein or Protein A. ### Claim 5 (depends on 1) The affinity support according to claim 1, wherein the protein having the affinity to an immunoglobulin comprises at least one immunoglobulin-binding domain selected from the group consisting of an immunoglobulin-binding domain consisting of the amino acid sequence of SEQ ID NO: 3, an immunoglobulin-binding domain comprising a partial sequence of the amino acid sequence of SEQ ID NO: 3, and an immunoglobulin-binding domain consisting of an amino acid sequence having at least 70% identity to the amino acid sequence of SEQ ID NO: 3. ### Claim 6 (depends on 4) The affinity support according to claim 4, wherein the protein having the affinity to an immunoglobulin comprises two or more of the immunoglobulin-binding domains. ### Claim 7 (depends on 1) The affinity support according to claim 1, wherein the solid phase support comprises at least one reactive group capable of binding a thiol group or an amino group. ### Claim 8 (depends on 7) The affinity support according to claim 7, wherein the at least one reactive group is an epoxy group. ### Claim 9 (depends on 1) A method for isolating immunoglobulin, comprising: isolating the immunoglobulin with the affinity support according to claim 1. ### Claim 10 An affinity ligand, comprising: a linker R and a protein ligand R1, wherein the linker R comprises a polyproline comprising from 12 to 24 proline residues, R1 is a protein having an affinity to an immunoglobulin, and R is bound to a C terminal or an N terminal of an amino acid sequence of the protein R1.