# US 10,766,829 B2 · Yeast extract having effect of promoting growth of plant and elongation of root and effect of improving added values of plant > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,766,829 B2 - Document ID: US10766829B2 - Status: Granted - Kind: B2 - Application number: 15/558,791 - Filed: 2016-03-16 - Published: 2018-03-15 - Granted: 2020-09-08 - Earliest claimed: 2015-03-19 - Assignees: Kohjin Life Sciences Co., Ltd. - Applicants: Kohjin Life Sciences Co., Ltd. - Inventors: Tomohiro Nakagawa; Yoshie Yasumatsu; Naoto Kaji; Kenichi Ason - Web page: https://patentgrep.com/patent/US10766829B2 ## CPC - C05F 11/08: Organic fertilisers containing added bacterial cultures, mycelia or the like - A01G 7/06: Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants - A01G 24/00: Growth substrates; Culture media; Apparatus or methods therefor (for mushrooms A01G18/20) - A01N 63/32: Yeast - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - A01N 63/60: Isolated nucleic acids - C05F 9/00: Fertilisers from household or town refuse - C05F 9/02: Apparatus for the manufacture - C05G 1/00: Mixtures of fertilisers belonging individually to different subclasses of C05 - C05G 5/23: Solutions - C12N 1/16: Yeasts; Culture media therefor - Y02A 40/20: Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses - C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C05G: MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS (organic fertilisers containing added bacterial cultures, mycelia, or the like C05F11/08; organic fertilisers containing plant vitamins or hormones C05F11/10); FERTILISERS CHARACTERISED BY THEIR FORM - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract [Problem] The present invention seeks to provide a composition for plants that is highly safe and that contributes to early harvesting, increasing yield, and increasing added value of crops. Specifically, the present invention provides a yeast extract that, by addition to a foliar surface spray or to soil or water, provides an effect of promoting growth, an effect of root lengthening, an effect of improved taste, and an effect of increased amino acid content of a plant. A substance obtained from yeast that is edible and considered to be safe is preferred as the yeast extract. [Means for Solving the Problem] A yeast extract having a peptide content of 5 wt % or more and an RNA content of 5 wt % or more is sprayed onto or provided as fertilizer to a plant. The yeast extract is preferably derived from Candida utilis, which is edible and considered to be safe. ## Claims ### Claim 1 A foliar surface spray liquid comprising: a yeast extract having a peptide content of 5 wt % or more and an RNA content of 5 wt % or more, wherein the yeast extract in the foliar surface spray liquid is at a dry weight of between 1.0 ppm and 100,000 ppm. ### Claim 2 (depends on 1) The foliar surface spray liquid according to claim 1, wherein the yeast extract has a peptide content of 15 wt % or more, an RNA content of 25 wt % or more, and a free amino acid content of 4 wt % or less. ### Claim 3 (depends on 1) The foliar surface spray liquid according to claim 1, wherein the yeast extract is extracted from Candida utilis cells. ### Claim 4 (depends on 1) The foliar surface spray liquid according to claim 1, wherein the yeast extract is extracted from Saccharomyces cerevisiae cells. ### Claim 5 (depends on 1) The foliar surface spray liquid according to claim 1, further comprising a fertilizer. ### Claim 6 (depends on 5) The foliar surface spray liquid according to claim 5, wherein the fertilizer is a chemical fertilizer or an organic fertilizer. ### Claim 7 (depends on 6) The foliar surface spray liquid according to claim 6, wherein the chemical fertilizer is nitrogen, phosphorus, or potassium. ### Claim 8 (depends on 6) The foliar surface spray liquid according to claim 6, wherein the organic fertilizer is a plant oil cake, a fish meal, or a powdered marine algae. ### Claim 9 A method of cultivating a plant, comprising: spraying onto a leaf surface of a plant, or adding to soil or a hydroponic solution used in plant cultivation, a composition for plants containing a yeast extract having a peptide content of 5 wt % or more and an RNA content of 5 wt % or more. ### Claim 10 (depends on 1, 9) The method of cultivating a plant according to claim 9, wherein the yeast extract in the foliar surface spray liquid according to claim 1 is at a concentration of 100 mg/L to 300 mg/L. ### Claim 11 (depends on 9) The method of cultivating a plant according to claim 9, wherein the plant is a tomato. ### Claim 12 (depends on 9) The method of cultivating a plant according to claim 9, wherein the plant is a Japanese mustard spinach. ### Claim 13 (depends on 9) The method of cultivating a plant according to claim 9, wherein the plant is a Japanese radish leaf. ### Claim 14 (depends on 1, 9) The method of cultivating a plant according to claim 9, wherein the plant is a tomato, and wherein the yeast extract in the foliar surface spray liquid according to claim 1 is at a concentration of 300 mg/L. ### Claim 15 (depends on 1, 9) The method of cultivating a plant according to claim 9, wherein the plant is a Japanese mustard spinach, and wherein the yeast extract in the foliar surface spray liquid according to claim 1 is at a concentration of 200 mg/L or more. ### Claim 16 (depends on 1, 9) The method of cultivating a plant according to claim 9, wherein the plant is a Japanese radish leaf, and wherein the yeast extract in the foliar surface spray liquid according to claim 1 is at a concentration of 200 mg/L or less. ### Claim 17 (depends on 9) The method of cultivating a plant according to claim 9, wherein the plant is from the family Brassicaceae.