Back to results
US 10,766,798 B2Granted
Engineered yeast as a method for bioremediation
AssigneeMassachusetts Institute of Technology
InventorsGeorge Le-Le Sun, Angela M. Belcher
Life of this patent
Estimating the term…
Abstract
Metal bioremediation and metal mining strategies can include compositions and methods.
Claims33
A composition for remediating a metal to treat water comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal, wherein the metal binding protein is fused with a high affinity protein including a metal binding domain, wherein the metal binding domain has specificity for the metal.
4 more elements
A method of remediating a metal to treat water comprising:
- preparing a composition comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal, wherein the metal binding protein is fused with a high affinity protein including a metal binding domain, wherein the metal binding domain has specificity for the metal;
- and
- contacting water with the composition.
7 more elements
A method of making a composition for remediating a metal to treat water comprising:
- selecting a cell;
- expressing a metal binding protein on a surface of the cell;
- and
- tethering a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal, wherein the metal binding protein is fused with a high affinity protein including a metal binding domain, wherein the metal binding domain has specificity for the metal.
5 more elements
A method of mining a metal comprising:
- preparing a composition comprising:
- a cell;
- and
- a first oligomer of a metal binding protein expressed on a surface of the cell via a linker;
- wherein the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell;
- wherein the metal binding protein has specificity for a metal;
- and
- wherein the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal, wherein the metal binding protein is fused with a high affinity protein including a metal binding domain, wherein the metal binding domain has specificity for the metal;
- contacting water with the composition;
- and
- lysing the cell to obtain the metal.
8 more elements
Dependencies are inferred from the claim text.