# US 10,765,971 B2 · Three-port chamber for processing particles > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,765,971 B2 - Document ID: US10765971B2 - Status: Granted - Kind: B2 - Application number: 14/827,130 - Filed: 2015-08-14 - Published: 2016-02-18 - Granted: 2020-09-08 - Earliest claimed: 2014-08-14 - Assignees: Terumo BCT, Inc. - Applicants: Terumo BCT, Inc. - Inventors: Timothy Michael Taga; Anne Baker Oram; Linda A. Taylor; Dennis J. Hlavinka - Web page: https://patentgrep.com/patent/US10765971B2 ## CPC - B01D 21/262: {by using a centrifuge} - B04B 5/0442: {with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation} - B04B 2005/0471: {with additional elutriation separation of different particles} - C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)} - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - B04B: CENTRIFUGES (high-speed drum mills B02C19/11; domestic spin driers D06F; analysing, measuring or monitoring physical or chemical properties of samples during centrifuging, see the relevant subclasses for these procedures, e.g. G01N) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract Embodiments relate to systems, chambers, and methods for processing particles by washing, concentrating, and/or treating the particles. Some embodiments provide for the processing of cells that may be in a liquid medium (e.g., a liquid in which the cells were grown) and are processed by being washed and concentrated for later use in research or other therapies. ## Claims ### Claim 1 A chamber for separating particles from a liquid, the chamber comprising: a first side wall defining a first volume, wherein the first volume comprises a first cross-sectional area and a second cross-sectional area, the second cross-sectional area being smaller than the first cross-sectional area; a second side wall defining a second volume located above the first volume; a first port in fluid communication with the first volume, wherein the first port is located in the first side wall; a first wall adjacent the first port, the first wall, said first wall being curved and preventing flow of particles in liquid into the second volume, wherein the first wall at least partially defines a channel for flow of particles away from the second volume; a second port in fluid communication with the first volume, wherein the second port is located below the first port; and a third port in fluid communication with the second volume, wherein the third port is located above the second port. ### Claim 2 (depends on 1) The chamber of claim 1, wherein at least a portion of the first side wall is angled with respect to a central axis. ### Claim 3 (depends on 2) The chamber of claim 2, wherein the at least a portion of the first side wall defines a conical volume that makes up the first volume. ### Claim 4 (depends on 3) The chamber of claim 3, wherein the first port is located near the first cross-sectional area. ### Claim 5 (depends on 3) The chamber of claim 3, wherein the channel and first wall direct particles and liquid, introduced through the first port, toward an inside surface of the first side wall. ### Claim 6 (depends on 1) The chamber of claim 1, further comprising a second wall located diametrically opposite the first wall. ### Claim 7 (depends on 6) The chamber of claim 6, wherein the second wall is curved. ### Claim 8 (depends on 6) The chamber of claim 6, wherein the second wall further defines the channel for flow of particles away from the second volume. ### Claim 9 (depends on 6) The chamber of claim 6, wherein the first wall and the second wall are part of a continuous skirt. ### Claim 10 (depends on 9) The chamber of claim 9, wherein the channel directs particles and liquid in a direction tangential to a portion of the first side wall. ### Claim 11 (depends on 10) The chamber of claim 10, wherein the channel comprises a partial wall that aids in directing particles and liquid in a direction tangential to a portion of the first side wall. ### Claim 12 (depends on 9) The chamber of claim 9, further comprising an entry pathway in fluid communication with the first port and the channel. ### Claim 13 (depends on 12) The chamber of claim 12, wherein the entry pathway directs particles and liquid toward a sloped surface. ### Claim 14 (depends on 13) The chamber of claim 13, wherein the sloped surface is part of the first port. ### Claim 15 (depends on 2) The chamber of claim 2, wherein the third port is positioned in a top wall that further defines the second volume. ### Claim 16 (depends on 15) The chamber of claim 15, wherein the top wall is angled with respect to the central axis. ### Claim 17 (depends on 16) The chamber of claim 16, wherein the third port is co-linear with the central axis. ### Claim 18 (depends on 2) The chamber of claim 2, wherein the first port is an inlet for introducing particles and liquid into the volume. ### Claim 19 (depends on 18) The chamber of claim 18, wherein the second port is an outlet for removing particles from the volume. ### Claim 20 (depends on 19) The chamber of claim 19, wherein the second port is an inlet for introducing liquid into the volume. ### Claim 21 (depends on 20) The chamber of claim 20, wherein the third port is an outlet for removing liquid from the volume.