# US 10,765,780 B2 · Method of producing tissue regeneration substrate

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- Number: US 10,765,780 B2
- Document ID: US10765780B2
- Status: Granted
- Kind: B2
- Application number: 15/524,922
- Filed: 2015-12-03
- Published: 2017-11-02
- Granted: 2020-09-08
- Earliest claimed: 2014-12-05
- Assignees: Gunze Limited
- Applicants: Gunze Limited
- Inventors: Hidetoshi Arimura; Keita Ide
- Web page: https://patentgrep.com/patent/US10765780B2

## CPC

- A61L 27/56: Porous materials, {e.g. foams or sponges}
- A61F 2/06: Blood vessels
- A61F 2/105: {Skin implants, e.g. artificial skin}
- A61F 2/28: Bones
- A61F 2/30756: {Cartilage endoprostheses (A61F2/3603 takes precedence)}
- A61L 27/18: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- A61L 27/34: Macromolecular materials
- A61L 27/58: Materials at least partially resorbable by the body
- B32B 5/022: {Non-woven fabric}
- D01D 5/0985: {by means of a flowing gas (e.g. melt-blowing)}
- A61F 2002/30062: {(bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive}
- A61F 2210/0004: bioabsorbable
- A61L 2430/02: for reconstruction of bones; weight-bearing implants
- B32B 2250/02: 2 layers
- B32B 2250/20: All layers being fibrous or filamentary
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C)
- B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- D01D: MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS

## Abstract

The present invention aims to provide a tissue regeneration substrate excellent in penetrability to cells as well as capable of effectively preventing cell leakage from the tissue regeneration substrate to accelerate tissue regeneration; and a method of producing the tissue regeneration substrate. The present invention relates to a tissue regeneration substrate including: a nonwoven fabric made of a bioabsorbable material, the tissue regeneration substrate having a laminated structure in which a layer containing a nonwoven fabric having an average pore size of 20 to 50 μm and a layer containing a nonwoven fabric having an average pore size of 5 to 20 μm are integrated.

## Claims

### Claim 1

A method of producing a tissue regeneration substrate comprising:
preparing a layer containing a nonwoven fabric having an average pore size of 20 to 50 μm;
discharging threads made of a bioabsorbable material on the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm by melt blowing to form a layer containing a nonwoven fabric having an average pore size of 5 to 18 μm to produce a stack; and
needle-punching the stack to integrate the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm and the layer containing a nonwoven fabric having an average pore size of 5 to 18 μm.

### Claim 2

A method of producing a tissue regeneration substrate comprising:
preparing a layer containing a nonwoven fabric having an average pore size of 20 to 50 μm;
preparing a layer containing a nonwoven fabric having an average pore size of 5 to 18 μm;
stacking the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm and the layer containing a nonwoven fabric having an average pore size of 5 to 18 μm to produce a stack; and
needle-punching the stack to integrate the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm and the layer containing a nonwoven fabric having an average pore size of 5 to 18 μm.

### Claim 3 (depends on 1)

The method of producing the tissue regeneration substrate according to claim 1, further comprising, after preparing the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm, fluffing a surface of the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm on which the layer containing a nonwoven fabric having an average pore size of 5 to 18 μm is to be stacked.

### Claim 4 (depends on 2)

The method of producing the tissue regeneration substrate according to claim 2, further comprising, after preparing the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm, fluffing a surface of the layer containing a nonwoven fabric having an average pore size of 20 to 50 μm on which the layer containing a nonwoven fabric having an average pore size of 5 to 18 μm is to be stacked.