# US 10,765,735 B2 · Adjuvant compositions and methods of use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,765,735 B2 - Document ID: US10765735B2 - Status: Granted - Kind: B2 - Application number: 15/045,986 - Filed: 2016-02-17 - Published: 2016-06-09 - Granted: 2020-09-08 - Earliest claimed: 2009-01-05 - Assignees: EpitoGenesis, Inc. - Applicants: EpitoGenesis, Inc. - Inventors: Michael Vajdy; Shohre Golestani - Web page: https://patentgrep.com/patent/US10765735B2 ## CPC - A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants - A61K 39/12: Viral antigens - A61K 39/145: Orthomyxoviridae, e.g. influenza virus - A61K 39/21: Retroviridae, e.g. equine infectious anemia virus - A61P 31/16: for influenza or rhinoviruses - A61P 37/02: Immunomodulators - A61P 37/04: Immunostimulants - C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) - A61K 2039/541: {Mucosal route} - A61K 2039/543: {intranasal} - A61K 2039/55511: {Organic adjuvants} - A61K 2039/55588: {Adjuvants of undefined constitution} - C12N 2740/16034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C12N 2740/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C12N 2740/16334: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C12N 2760/16034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C12N 2760/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C12N 2760/16151: Methods of production or purification of viral material - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract This disclosure provides adjuvant compositions that are capable of modulating the immune response in a subject. These adjuvant compositions may also be used enhance the immunogenicity of antigens. Also provided are methods of making the adjuvant compositions as well as methods of using the adjuvant compositions. ## Claims ### Claim 1 An adjuvant comprising: (a) a pharmaceutically acceptable oil selected from the group consisting of wintergreen oil, rosemary oil, mustard seed oil, allyl isothiocyanate, and a mixture thereof; (b) at least one catechin; and (c) at least one vitamin selected from the group consisting of vitamin A, vitamin E and vitamin D; wherein the adjuvant does not comprise an antigen. ### Claim 2 (depends on 1) The adjuvant of claim 1, further comprising one or more emulsifiers. ### Claim 3 (depends on 2) The adjuvant of claim 2, wherein the one or more emulsifiers are selected from the group consisting of a polysorbate, phospholipids, sorbitan monolaureate, sorbitan monopalmitate, sorbitan monostearate, sorbitan tristearate, sorbitan monooleate, sorbitan trioleate, and a combination thereof. ### Claim 4 (depends on 1) The adjuvant of claim 1, wherein said catechin is epigallocatechin gallate (EGCG). ### Claim 5 (depends on 1) The adjuvant of claim 1, wherein said at least one vitamin is vitamin E. ### Claim 6 (depends on 1) The adjuvant of claim 1, wherein said at least one vitamin is vitamin A. ### Claim 7 (depends on 1) The adjuvant of claim 1, wherein said at least one vitamin is vitamin D. ### Claim 8 (depends on 1) The adjuvant of claim 1, wherein the pharmaceutically acceptable oil is mustard seed oil. ### Claim 9 (depends on 1) The adjuvant of claim 1, wherein the pharmaceutically acceptable oil is allyl isothiocyanate. ### Claim 10 (depends on 1) The adjuvant of claim 1, wherein the pharmaceutically acceptable oil is wintergreen oil. ### Claim 11 (depends on 1) The adjuvant of claim 1, wherein the pharmaceutically acceptable oil is rosemary oil. ### Claim 12 (depends on 1) A method of modulating an immune response in a subject consisting of administering the adjuvant of claim 1 to said subject. ### Claim 13 (depends on 12) The method of claim 12, wherein the subject is a mammal. ### Claim 14 An immunogenic composition comprising an adjuvant and an antigen, wherein the adjuvant comprises: (a) a pharmaceutically acceptable oil selected from the group consisting of wintergreen oil, rosemary oil, mustard seed oil, allyl isothiocyanate, and a mixture thereof; (b) at least one catechin; and (c) at least one vitamin selected from the group consisting of vitamin A, vitamin E and vitamin D. ### Claim 15 (depends on 14) The immunogenic composition of claim 14, wherein the antigen is derived from a virus, fungi, bacterium, prion, or cancer cell. ### Claim 16 (depends on 14) The immunogenic composition of claim 14, wherein the pharmaceutically acceptable oil is mustard seed oil. ### Claim 17 (depends on 14) The immunogenic composition of claim 14, wherein the pharmaceutically acceptable oil is allyl isothiocyanate. ### Claim 18 (depends on 14) The immunogenic composition of claim 14, wherein the pharmaceutically acceptable oil is wintergreen oil. ### Claim 19 (depends on 14) The immunogenic composition of claim 14, wherein the pharmaceutically acceptable oil is rosemary oil. ### Claim 20 (depends on 14) A method of modulating an immune response in a subject consisting of administering the immunogenic composition of claim 14 to said subject.