# US 10,765,715 B2 · Composition containing saponins of Panax ginseng as active ingredient

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- Number: US 10,765,715 B2
- Document ID: US10765715B2
- Status: Granted
- Kind: B2
- Application number: 16/062,101
- Filed: 2016-10-28
- Published: 2018-12-27
- Granted: 2020-09-08
- Earliest claimed: 2015-12-14
- Assignees: Korean Drug Co., Ltd.
- Applicants: Kyungsung University Industry Cooperation Foundation
- Inventors: Jae Seon Kang; Bo Suel Kim; Heyong-Soo Kim; Gyu-Jin Rho; Sin-Ja Bae; Seo Hyun Lee; Jong Jin Park; Choel-Min Kim
- Web page: https://patentgrep.com/patent/US10765715B2

## CPC

- A61K 36/258: Panax (ginseng)
- A23L 33/10: using additives (addition of substantially indigestible substances A23L33/21)
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61K 31/575: substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
- A61K 31/704: attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin {(digitoxin A61K31/7048)}
- A61P 3/06: Antihyperlipidemics
- A61P 7/06: Antianaemics
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A61K 2236/15: involving mechanical treatment, e.g. chopping up, cutting or grinding
- A61K 2236/333: using mixed solvents, e.g. 70% EtOH
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- A61K 2236/53: Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Disclosed are uses of ginseng saponin, which contains at least 90% of a compound K, Rd, F2, and Rg3 as a main ingredient and has effects in extending the lifespan of the cell, promoting the cell differentiation, increasing the number of red blood cells, and reducing the triglycerides by extracting, heat-treating, and enzyme-converting the ginseng saponin to prepare active saponins Rd, F2, and Rg3 including a compound K.

## Claims

### Claim 1

A composition containing ginseng saponin as an active ingredient which is useful for extending the lifespan of cells and promoting differentiation of cells, wherein the ginseng saponin is prepared by:
exposing the ginseng to steam under a pressure of 1.1 kg/cm2 while removing moisture at 121° C. for 10 minutes using an automatic temperature and humidity control device with a porous shelf (step S1);
continuously injecting air into the ginseng subjected to the step S1 and cooling the ginseng to 90° C. or lower (step S2);
repeating the steps S1 and S2 two times (step S3);
cooling the ginseng to room temperature in an automatic temperature control device and pulverizing the ginseng to a particle size of 1 mm or less, after step S3 (step S4);
extracting the saponin by adding 80% ethyl alcohol to the ginseng subjected to the step S4, and concentrating the extracted saponin (step S5);
preparing a suspension by dissolving the concentrated saponin in ethanol and adding sterilized water, after step S5 (step S6);
adding the suspension to and reacting the suspension with a pectinase enzyme solution drop by drop, after step S6 (step S7);
precipitating the solution subjected to the reaction of step S7 by centrifugation (step S8);
dissolving the generated precipitate in ethyl alcohol (step S9);
suspending the dissolved precipitate by adding sterilized physiological saline solution (step (10);
filtering the suspended precipitate (step S11); and
resuspending the precipitate in sterile physiological saline solution (step S12).

### Claim 2 (depends on 1)

The composition of claim 1, wherein when the composition is used in a method to extend the lifespan of the cells, the life span extension is suppressed when the cells are contacted by an effective amount of β-galactosidase.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the promoting of the differentiation of cells is promoting of differentiation of stem cells.

### Claim 4

A composition containing ginseng saponin as an active ingredient which is useful for reducing triglycerides and increasing the number of red blood cells, wherein the ginseng saponin is prepared by,
exposing the ginseng to steam under a pressure of 1.1 kg/cm2 while removing moisture at 121° C. for 10 minutes using an automatic temperature and humidity control device with a porous shelf (step S1);
continuously injecting air into the ginseng subjected to the step S1 and cooling the ginseng to 90° C. or lower (step S2);
repeating the steps S1 and S2 two times (step S3);
cooling the ginseng to room temperature in an automatic temperature control device and pulverizing the ginseng to a particle size of 1 mm or less, after step S3 (step S4);
extracting the saponin by adding 80% ethyl alcohol to the ginseng subjected to the step S4, and concentrating the extracted saponin (step S5);
preparing a suspension by dissolving the concentrated saponin in ethanol and adding sterilized water, after step S5 (step S6);
adding the suspension to and reacting the suspension with a pectinase enzyme solution drop by drop, after step S6 (step S7);
precipitating the solution subjected to the reaction of step S7 by centrifugation (step S8);
dissolving the generated precipitate in ethyl alcohol (step S9);
suspending the dissolved precipitate by adding sterilized physiological saline solution (step (10);
filtering the suspended precipitate (step S11); and
resuspending the precipitate in sterile physiological saline solution (step S12).

### Claim 5 (depends on 1)

The composition of claim 1, wherein the ginseng saponin contains 90% or higher of protopanaxadiol-based saponin.

### Claim 6 (depends on 5)

The composition of claim 5, wherein the protopanaxadiol-based saponin includes the compounds K, Rd, F2, and Rg3 which are active protopanaxadiols.

### Claim 7 (depends on 4)

The composition of claim 4, wherein the ginseng saponin contains 90% or higher of protopanaxadiol-based saponin.