# US 10,765,622 B2 · Process for the production of a composition for protecting skin from drying and/or UV damage and/or inflammation

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- Number: US 10,765,622 B2
- Document ID: US10765622B2
- Status: Granted
- Kind: B2
- Application number: 16/092,869
- Filed: 2017-03-30
- Published: 2019-05-23
- Granted: 2020-09-08
- Earliest claimed: 2016-04-14
- Assignees: Conopco, Inc.
- Applicants: Conopco, Inc.
- Inventors: Mark John Berry; Ravine Anthony Gungabissoon
- Web page: https://patentgrep.com/patent/US10765622B2

## CPC

- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61K 8/97: from algae, fungi, lichens or plants; from derivatives thereof
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/16: Emollients or protectives, e.g. against radiation
- A61Q 17/04: Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
- A61Q 19/007: {Preparations for dry skin}
- C12N 5/04: Plant cells or tissues {(culture media C12N5/0025)}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A process for the production of a Camellia sinensis dedifferentiated stem cell extract, the process comprising the steps of: (a) Preparing a cell culture comprising Camellia sinensis dedifferentiated stem cells; (b) Performing an extraction on the cell culture using ethanol and/or methanol as an extraction solvent, to produce the Camellia sinensis dedifferentiated stem cell extract.

## Claims

### Claim 1

A process for the production of a Camellia sinensis dedifferentiated stem cell extract, the process comprising the steps of:
(a) preparing a cell culture comprising Camellia sinensis dedifferentiated stem cells; and
(b) performing a one-step extraction of the cell culture using only ethanol and/or methanol as an extraction solvent, to produce the Camellia sinensis dedifferentiated stem cell extract.

### Claim 2 (depends on 1)

The process according to claim 1, wherein the cell culture is produced by culturing Camellia sinensis plant callus cells.

### Claim 3 (depends on 1)

The process according to claim 1, wherein the cell culture medium comprises the Hormones 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA) and 6-Benzylamino purine (BAR).

### Claim 4 (depends on 1)

The process according to claim 1, wherein the cell culture medium has a pH of from 5.6 to 6.0.

### Claim 5 (depends on 1)

A composition comprising a therapeutically effective amount of the Camellia sinensis dedifferentiated stem cell extract obtainable by the process according to claim 1.

### Claim 6 (depends on 5)

The composition according to claim 5, wherein the composition is an oral or topical skin treatment composition.

### Claim 7 (depends on 5)

The composition according to claim 5, wherein the composition comprises at least 0.01 wt % of the Camellia sinensis dedifferentiated stem cell extract.

### Claim 8 (depends on 7)

The composition according to claim 7, wherein the composition comprises at least 0.1 wt % of the Camellia sinensis stem dedifferentiated cell extract.