# US 10,765,111 B1 · Cryosolutions and uses thereof

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- Number: US 10,765,111 B1
- Document ID: US10765111B1
- Status: Granted
- Kind: B1
- Application number: 14/638,881
- Filed: 2015-03-04
- Granted: 2020-09-08
- Earliest claimed: 2014-03-05
- Assignees: Akron Biotechnology, LLC
- Applicants: Akron Biotechnology, LLC
- Inventors: Claudia Zylberberg
- Web page: https://patentgrep.com/patent/US10765111B1

## CPC

- A01N 1/125: Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

Compositions for maintaining cellular integrity especially in cases of cryopreservation and multiple freeze/thawing cycles are provided. In addition, the compositions can be used in various media for a variety of applications.

## Claims

### Claim 1

A composition for cryopreservation consisting of: polyethylene glycol (PEG), a saccharide, and an organic amphoteric agent, wherein the polyethylene glycol is present in the composition from 0.001% to 20% v/v, the saccharide is present in the composition from 0.1 μg/ml to 1 mg/ml and the organic amphoteric agent is present in the composition from 0.1% to 20% v/v, wherein:
said organic amphoteric agent is ectoin and/or hydroxyectoin;
said saccharide is trehalose and/or dextran-40;
said composition being added to a biological medium.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the composition consists of from 0.1 μg/ml to 0.9 mg/ml of trehalose and/or dextran-40, from 0.1% to 20% v/v ectoin and/or hydroxyectoin and from 0.001% to 20% v/v of PEG.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 800 μg/ml of trehalose.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 700 μg/ml of trehalose.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 500 μg/ml of trehalose.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 250 μg/ml of trehalose.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 100 μg/ml of trehalose.

### Claim 8 (depends on 1)

The composition of claim 1, wherein the composition comprises from 0.1 μg/ml to 50 μg/ml of trehalose.