# US 10,765,110 B2 · Agent for preserving biological component

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- Number: US 10,765,110 B2
- Document ID: US10765110B2
- Status: Granted
- Kind: B2
- Application number: 15/524,057
- Filed: 2015-11-10
- Published: 2017-11-02
- Granted: 2020-09-08
- Earliest claimed: 2014-11-11
- Assignees: Yasuhiko Tabata; Denka Company Limited
- Applicants: Yasuhiko Tabata; Denka Company Limited
- Inventors: Yasuhiko Tabata; Kenji Fujii; Masamichi Hashimoto; Akio Ohno
- Web page: https://patentgrep.com/patent/US10765110B2

## CPC

- A01N 1/122: Preservation or perfusion media
- A01N 1/128: Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12N 5/00: Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided is an agent for preserving a biological component, comprising a hydrogel, the hydrogel comprising a crosslinked product formed from a compound having a plurality of hydroxyl groups and a modified hyaluronic acid having substituents capable of reacting with the hydroxyl groups to form a crosslinked structure.

## Claims

### Claim 1

An agent for preserving a biological component, comprising a hydrogel, the hydrogel comprising a crosslinked product formed from polyvinyl alcohol and a modified hyaluronic acid having substituents capable of reacting with the hydroxyl groups to form a crosslinked structure;
wherein
the modified hyaluronic acid has disaccharide units and is represented by the following formula (1):
wherein R1 represents a direct bond or a divalent group; and
wherein the hydrogel further comprises unmodified hyaluronic acid.

### Claim 2 (depends on 1)

The agent according to claim 1, wherein the hydrogel further comprises at least one selected from a medium for cells and a physiological buffer.

### Claim 3 (depends on 1)

The agent according to claim 1, wherein the biological component comprises at least one selected from cells, a 2-dimensional or 3-dimensional cellular aggregate constructed from cells, and a tissue or organ of biological origin.

### Claim 4

A kit for preparing an agent for preserving a biological component, the kit comprising a first agent containing polyvinyl alcohol and unmodified hyaluronic acid and a second agent containing a modified hyaluronic acid having substituents capable of reacting with the hydroxyl groups to form a crosslinked structure;
wherein
the modified hyaluronic acid has disaccharide units and is represented by the following formula (1):
wherein R1 represents a direct bond or a divalent group.