Image-processing device and cell observation system
AssigneeOlympus Corporation
InventorsTakayuki Nakatomi, Satoshi Ishii, Isao Sakane, Masakazu Fujii, Akira Matsushita, Takashi Miyoshi
Life of this patent
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Abstract
Provided is an image-processing device that includes: a processor including hardware; the processor configured to: analyze an image of the inside of a culture vessel that cultures cells, and acquire quantitative data of living cells and quantitative data of dead cells inside the culture vessel; and generate comparison data that allows comparison of the acquired quantitative data of the living cells and the acquired quantitative data of the dead cells.
Claims14
A cell observation system comprising:
- a culture observation device that acquires, images of cells being cultured inside a culture vessel;
- a computer that is configured to perform steps (i)-(iii), in order, a plurality of times:
- (i) acquire an image;
- (ii) analyze the image acquired by the culture observation device, and acquire quantitative data of living cells and quantitative data of dead cells inside the culture vessel;
- and
- (iii) record the acquired quantitative data of living cells and quantitative data of dead cells in a database;
- and, the computer further configured to
- generate comparison data that allows comparison of changes in the acquired quantitative data of the living cells and changes in the acquired quantitative data of the dead cells;
- and
- a monitor that displays the generated comparison data.
A cell observation system comprising:
- a culture observation device that acquires an image of inside of a culture vessel that cultures cells;
- a computer that is configured to:
- analyze the image acquired by the culture observation device, and acquire quantitative data of living cells and quantitative data of dead cells inside the culture vessel;
- and
- generate comparison data that allows comparison of the acquired quantitative data of the living cells and the acquired quantitative data of the dead cells;
- a monitor that displays the generated comparison data;
- and
- a recording device that records the acquired quantitative data,
- wherein the recording device has reference data of changes in the quantitative data of the living cells over time and reference data of changes in the quantitative data of the dead cells over time for each cell type,
- the computer is configured to generate comparison data that allows comparison of a graph of changes in the quantitative data of the living cells over time and the reference data of the living cells, and that allows comparison of a graph of changes in the quantitative data of the dead cells over time and the reference data of the dead cells,
- the reference data includes a lower limit and an upper limit set for the quantitative data of the living cells, and a lower limit and an upper limit set for the quantitative data of the dead cells, and
- the computer is configured to compare the quantitative data of the living cells with the lower limit and the upper limit therefor, compare the quantitative data of the dead cells with the lower limit and the upper limit therefor, and determine whether or not a condition of the cells is acceptable on a basis of results of the comparisons.
A non-transitory computer-readable medium having a program stored therein, the program causing a computer to:
- perform steps (i)-(iii), in order, a plurality of times,
- (i) acquiring an image;
- (ii) analyzing the image of cells being cultured inside a culture vessel, the image being acquired over time, and acquiring quantitative data of living cells and quantitative data of dead cells inside the culture vessel;
- (iii) recording the acquired quantitative data of living cells and quantitative data of dead cells in a database,
- the program causing the computer to also
- generate comparison data that allows comparison of changes in the acquired quantitative data of the living cells over time and changes in the acquired quantitative data of the dead cells over time;
- and
- output the generated comparison data to be displayed.
Dependencies are inferred from the claim text.