Method for derivatizing an s-cis-diene compound, derivatization reagent kit, and method for analyzing an s-cis-diene compound
AssigneesTokyo University of Science, National University Corporation Chiba University, JEOL Ltd.
InventorsTatsuya Higashi, Shoujiro Ogawa, Fumio Nomura, Mamoru Satoh, Masaki Takiwaki
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Abstract
Provided is a method for derivatizing an s-cis-diene compound with a Cookson-type derivatization reagent, the method including adding, in a reaction stopping step of stopping a derivatization reaction of the s-cis-diene compound, a decomposition inhibitor to inhibit decomposition of a derivative to be obtained.
Claims5
A method for derivatizing an s-cis-diene compound with a Cookson-type derivatization reagent,
- the method comprising adding, in a reaction stopping step of stopping a derivatization reaction of the s-cis-diene compound, a decomposition inhibitor to inhibit decomposition of a derivative to be obtained, and
- evaporating solvent after completion of the reaction stopping step to form a residue of the derivatized s-cis-diene compound,
- wherein the Cookson-type derivatization reagent is 4-(4′-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD), the s-cis-diene compound is vitamin D or a vitamin D metabolite, and the decomposition inhibitor is trimethylamine.
A derivatization reagent kit to derivatize an s-cis-diene compound with a Cookson-type derivatization reagent and form a residue of the derivatized s-cis-diene compound,
- the derivatization reagent kit comprising:
- a Cookson-type derivatization reagent;
- a reaction stopping agent to stop a derivatization reaction of the s-cis-diene compound;
- and
- a decomposition inhibitor to inhibit decomposition of a derivative to be obtained,
- wherein the Cookson-type derivatization reagent is 4-(4′-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD), the s-cis-diene compound is vitamin D or a vitamin D metabolite, and the decomposition inhibitor is trimethylamine.
Dependencies are inferred from the claim text.