Sandwich assays in droplets
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Darren Roy Link
Life of this patent
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Abstract
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
Claims18
A reagent library of a plurality of stabilized fluidic compartments in a container, each fluidic compartment comprising:
- a first binding agent specific for a first binding region on a target analyte, the first binding agent comprising a target identifier;
- and
- a second binding agent specific for a second binding region on the target analyte, wherein the second binding agent comprises a functionalized portion for immobilization to a solid support;
- wherein the target identifier in each respective fluidic compartment is unique;
- and
- wherein the fluidic compartments do not contain the solid support.
Dependencies are inferred from the claim text.