Method for determining a protein-protein interaction
AssigneeMedical & Biological Laboratories Co., Ltd.
InventorsTaku Watanabe, Toru Shoji, Shun Matsuzawa
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Abstract
A method for determining an interaction between a first protein and a second protein comprises the steps of: expressing in a cell or introducing into a cell a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and a second fusion protein comprising the second protein and a multimerizable protein; detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; and determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus.
Claims4
A method for determining an interaction between a first protein and a second protein, the method comprising the following steps (1) to (3):
- (1) expressing in a cell or introducing into a cell
- a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and
- a second fusion protein comprising the second protein and a multimerizable protein;
- (2) detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell;
- and
- (3) determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus,
- wherein the multimerizable protein in said first fusion protein, and the multimerizable protein in said second fusion protein, are the same, and
- wherein the multimerizable protein is selected from the group consisting of PB1 domain of p62, a PB1 domain of TFG, a PB1 domain of PKCiota, a SAM domain of TEL, a SAM domain of DGK delta, and a SAM domain of Tankyrase-1.
Dependencies are inferred from the claim text.