Producing a separation medium using grayscale mask
AssigneeThe Regents of the University of California
InventorsChi-Chih Kang, Amy E. Herr, Todd A. Duncombe
Life of this patent
Estimating the term…
Abstract
Provided are methods for producing a separation medium, where the method includes exposing a separation medium precursor solution to light from a light source through a photomask that includes a region with varied light transmittance to produce the separation medium. Systems that find use in performing the methods, microfluidic devices that include the separation medium, as well as methods of using the microfluidic devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.
Claims7
A method for producing a spatially varied separation medium, the method comprising:
- exposing a separation medium precursor solution to light from a light source through a photomask to produce a separation medium,
- wherein the photomask comprises a semi-transparent region with varied light transmittance comprising a pattern of opaque regions and optically transmissive regions,
- wherein an optically transmissive region is positioned between opaque regions, wherein an opaque region is positioned between optically transmissive regions,
- wherein the separation medium has a density gradient, and
- wherein the separation medium precursor solution comprises an acrylamide monomer or derivative thereof, a crosslinker, and a photoinitiator.
Dependencies are inferred from the claim text.