Method of recalibrating a device for assessing concentration of at least one analyte ion in a liquid
AssigneeCRC CARE Pty Ltd
InventorsRavendra Naidu, Liang Wang, Zuliang Chen, Mallavarapu Megharaj
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Abstract
The present invention relates to a method of recalibrating a device for assessing concentration of at least one analyte ion in a liquid, the device having a plurality of ion selective electrodes (ISEs) generating a signal in response to sensing a selected ion in the liquid, and a data processing unit implementing a neural network algorithm that has been trained to calculate ion interference between the selected ion and other ions in the liquid sensed at one of the electrodes and/or electrode interference between ones of the electrodes sensing a same selected ion.
Claims11
A method of recalibrating a device for assessing concentration of at least one analyte ion in a liquid, the device having:
- a plurality of ion selective electrodes (ISEs) each of which generate a signal in response to sensing a selected ion in the liquid;
- and a data processing unit implementing a neural network algorithm that has been trained to calculate ion interference between the selected ion and other ions in the liquid sensed at one of the electrodes and/or electrode interference between ones of the electrodes sensing a same selected ion based on a result of a comparison of training data indicative of known ionic concentrations applied to the neural network algorithm and the known ionic concentrations, the method includes:
- contacting, with the plurality of electrodes, more than one recalibrating sample having n selected calibration ions and a plurality of interference calibration ions of known ionic concentrations, respectively, whereby each of the n selected calibration ions correspond to ones of the plurality of electrodes;
- and
- the data processing unit:
- receiving a signal from each of the electrodes in response to sensing an ionic concentration of a corresponding one of the n selected calibration ions and the plurality of interference calibration ions in the more than one recalibrating sample;
- simulating a response of each of the plurality of electrodes by applying the signal as recalibration training data to a recalibration algorithm that has been trained to simulate the response based on a comparison of the recalibration training data and the known ionic concentration of the n selected calibration ions and the plurality of interference calibration ions, wherein stimulating the response of each of the plurality of electrodes is expressed as:
- V
- ISE
- =
- V
- pC
- Selected
- +
- ∑
- i
- =
- 1
- n
-
- Δ
-
-
- V
- pC
- Selected
- -
- pC
- Interferon
- i
- V
- ISE
- ≈
- a
- ×
- pC
- Selected
- +
- b
- +
- ∑
- i
- =
- 1
- n
-
- c
- i
- ×
- e
- d
- i
- ×
- (
- pC
- Selected
- -
- pC
- Interferon
- i
- )
- wherein:
- VISE is potential response value of the first electrode (ISE) (Unit:
- mV);
- VpCSelected is potential value caused by the first selected calibration ion (Unit:
- mV);
- ΔV is potential difference of each one of the plurality of electrodes ISE's response caused by each of the plurality of interference calibration ions (Unit:
- mV);
- pCSelected is concentration of the first selected calibration ion (Unit:
- −lg M);
- pCInterferoni is concentration of the ith one of the interference calibration ions (Unit:
- −lg M);
- and
- a, b, ci and di are parameters for the ith one of the interference calibration ions to be determined;
- and
- recalibrating the device by determining and optimizing the parameters a, b, ci, and di based on the known ionic concentrations of the n selected calibration ions and the plurality of interference calibration ions in the more than one recalibration sample.
Dependencies are inferred from the claim text.