Image processing apparatus and computer-readable recording medium storing program

A non-transitory computer-readable recording medium storing a program for causing a computer to function as:

• a communication interface in which a fluorescent image and a morphological image are input, the fluorescent image representing expression of a plurality of kinds of biological substances in a tissue specimen in which a fluorescent bright spot represents expression of the biological substances, and the morphological image representing morphology of a cell in the tissue specimen and including a range common to the fluorescent image;
• and
• a controller comprising at least one processor which, when instructed by the program, extracts an observation target region from the morphological image,
• extracts the fluorescent bright spot from the fluorescent image, and
• calculates a statistical value from a number of the plurality of kinds of biological substances whose expression is represented by the fluorescent bright spots, wherein the statistical value is based on a sum of expression numbers for each biological substance.