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US 10,761,026 B2Granted
Methods to quantify an ion in a dental biofilm
AssigneeThe Procter & Gamble Company
InventorsYunming Shi, Swapna Basa, Ross Strand
Life of this patent
Estimating the term…
Abstract
A method of quantitating of ions in the extracellular polymer substances area of a dental biofilm is an effective way of assessing efficacy of oral care compositions.
Claims17
A method of quantitating an ion selected from the group consisting of calcium, stannous, zinc, silver, copper, cetylpyridinium chloride, fluoride, and combinations thereof in a defined extracellular polymer substance (“EPS”) area of dental biofilm comprising the steps:
- (a) treating the dental biofilm with an oral care composition comprising an ion source selected from the group consisting of stannous, calcium, zinc, cetylpyridinium chloride, silver, copper, fluoride, and combinations thereof;
- (b) labeling the treated dental biofilm with an EPS fluorescent probe;
- (c) labeling the treated dental biofilm with at least one ion fluorescent probe;
- (d) defining the EPS area of the dental biofilm by measuring fluorescent light emitted from the labeled EPS biofilm;
- and
- (e) quantitating the ion by measuring fluorescence light emitted from the labeled ion within the defined EPS area of the biofilm.
3 more elements
Dependencies are inferred from the claim text.