Biosynthesis of phenylpropanoids and phenylpropanoid derivatives
AssigneeEvolva SA
InventorsMichael Naesby, Ernesto Simón Vecilla, Michael Eichenberger, Beata Joanna Lehka, Walden Emil Bjørn-Yoshimoto, Niels Bjerg Jensen
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Abstract
Provided herein are methods and compositions for producing phenylpropanoid derivatives, such as chalcones and stilbenes, and dihydrophenylpropanoid derivatives, such as dihydrochalcones and dihydrostilbenes, in microorganisms. In particular, the disclosure provides recombinant microorganisms and methods of use thereof for producing phenylpropanoid derivative compounds and dihydrophenylpropanoid derivative compounds.
Claims20
A method of producing a chalcone compound or a stilbene compound, comprising growing a yeast cell capable of producing a phenylpropanoid or phenylpropanoid derivative compound in a culture medium,
- wherein said chalcone compound or stilbene compound is synthesized by the yeast cell, and
- wherein the yeast cell comprises a gene encoding a double-bond reductase polypeptide, wherein expression of the gene or activity of the double-bond reductase polypeptide encoded thereby is reduced or eliminated, and wherein the double-bond reductase polypeptide:
- (i) comprises SEQ ID NO:
- 22;
- (ii) comprises a polypeptide with at least 90% identity to SEQ ID NO:
- 22;
- (iii) comprises SEQ ID NO:
- 26;
- (iv) comprises a polypeptide with at least 90% identity to SEQ ID NO:
- 26;
- (v) is encoded by a nucleic acid sequence comprising SEQ ID NO:
- 7;
- (vi) is encoded by a nucleic acid sequence comprising a sequence with at least 90% identity to SEQ ID NO:
- 7;
- (vii) is encoded by a nucleic acid sequence comprising SEQ ID NO:
- 43;
- or
- (viii) is encoded by a nucleic acid sequence comprising a sequence with at least 90% identity to SEQ ID NO:
- 43.
Dependencies are inferred from the claim text.