Kits suitable for use in electrochemiluminescence methods and methods of effecting a specific-binding non-wash assay

A kit suitable for use in an electrochemiluminescence method, comprising (a) one or more containers configured to receive a sample, (b) in said one or more containers, an electrode, wherein said electrode comprises a binding domain of a binding reagent immobilized thereto, said binding domain configured to form a complex with a peptide or a protein of said sample, (c) a pH buffer comprising 75-150 mM ECL co-reactant, wherein said buffer has a pH of 7 to 8, has a concentration of less than 5 mM inorganic phosphate, and comprises 100-600 mM tris[hydroxymethyl]aminomethane (Tris), wherein said ECL co-reactant comprises a non-TPA tertiary amine, and wherein said electrode is configured to contact said pH buffer and said sample and is configured to apply electrochemical energy to said sample and said pH buffer.