Chiral analysis method of bound amino acid and chiral analysis system
AssigneeKagami Inc.
InventorsKenji Hamase, Shoto Ishigo, Yurika Miyoshi, Tadashi Ueda, Masashi Mita
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Abstract
A chiral analysis method of a bound amino acid includes a step of hydrolyzing a bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide; a step of separating, by chiral separation, a D-form and an L-form of an amino acid generated by the hydrolyzation; a step of generating fragments from the separated amino acid; and a step of selecting and analyzing a predetermined fragment that contains an α carbon and does not contain a side chain from the generated fragments, by mass spectrometry.
Claims14
A chiral analysis method of a bound amino acid comprising:
- a step of hydrolyzing a bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide;
- a step of separating, by chiral separation, a D-form and an L-form of an amino acid generated by the hydrolyzation;
- a step of generating fragments from the separated amino acid;
- and
- a step of selecting and analyzing a predetermined fragment that contains an α carbon and does not contain a side chain from the generated fragments, by mass spectrometry.
A chiral analysis system of a bound amino acid comprising:
- an chiral separation part that separates, by chiral separation, a D-form and an L-form of an amino acid generated by hydrolyzing the bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide;
- a fragment generation part that generates fragments from the separated amino acid;
- and
- a mass spectrometry part that selects and analyzes a predetermined fragment that contains an α carbon and does not contain a side chain from the generated fragments, by mass spectrometry.
Dependencies are inferred from the claim text.