An analysis method using an automatic analysis apparatus including an analysis port including a reaction container holding part that holds a reaction container storing a liquid mixture of a sample and a reagent, a light source that emits light to the liquid mixture stored in the reaction container held by the reaction container holding part, and a detector that detects light generated when the light is emitted from the light source to the liquid mixture, and a control unit that controls the analysis port, the method comprising:
- analyzing the sample based on information on the detected light,
- reflecting at least a part of the light emitted from the light source by a surface of an inner wall of the reaction container holding part, and
- controlling the automatic analysis apparatus so as to emit the light from the light source in a state where the reaction container is not held by the reaction container holding part, to detect the light reflected on the surface of the inner wall of the reaction container holding part by the detector as reflected light, and not to use the analysis port for analysis when a result of a detection of the reflected light shows that the detected reflected light is less than a first value determined in advance.