PQQ-sGDH mutant, polynucleotide and glucose detection biosensor
AssigneeLeadway (Hk) Limited
InventorsZhe Wang, Xin Bi, Riyong Qiu
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Abstract
The present invention provides a soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-sGDH) mutant, wherein the amino acid positions thereof correspond to a wild-type PQQ-sGDH sequence of Acinetobacter calcoaceticus as shown in SEQ ID NO 1 comprising one of the following group of mutations: A194F, a combined mutation based on A194F, a combined mutation based on Q192A or a combined mutation based on Q192S. Said PQQ-sGDH mutant has good glucose substrate specificity and significantly reduced cross-reactivity to maltose and the like, and is suitable for detecting glucose in a sample such as blood.
Claims19
A pyrroloquinoline quinone-dependent soluble glucose dehydrogenase (PQQ-sGDH) protein having at least 90% sequence identity to the entire sequence of SEQ ID NO:
- 1, wherein said PQQ-sGDH protein comprises the following mutations relative to SEQ ID NO:
- 1:
- an A194F substitution, or
- one substitution selected from Q192A or Q192S, and a substitution occurring at at least one of positions G99, S170, L193, Q270, A318, M365, T366, Y367, T372, R402, N452 and K455.
Dependencies are inferred from the claim text.