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US 10,752,888 B2Granted
Method for enzymatically preparing highly concentrated myo-inositol
AssigneeCj Cheiljedang Corporation
InventorsSungjae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim, Seong Jun Cho
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Abstract
The present disclosure relates to a method for preparing myo-inositol using myo-inositol monophosphate synthase consisting of an amino acid sequence of SEQ ID NO: 1 and/or myo-inositol monophosphate phosphatase consisting of an amino acid sequence of SEQ ID NO: 3.
Claims8
A method for preparing myo-inositol, comprising:
- converting myo-inositol monophosphate to myo-inositol by reacting the myo-inositol monophosphate with myo-inositol monophosphate phosphatase consisting of the amino acid sequence of SEQ ID NO:
- 3, a microorganism expressing the same, or a culture of the microorganism.
A method for preparing myo-inositol, comprising reacting starch, maltodextrin, sucrose or a combination thereof, and phosphate with (a) myo-inositol monophosphate phosphatase consisting of the amino acid sequence of SEQ ID NO:
- 3;
- myo-inositol monophosphate synthase consisting of the amino acid sequence of SEQ ID NO:
- 1;
- phosphoglucomutase or glucokinase;
- and α-glucanophosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, α-amylase, pullulanase, isoamylase, glucoamylase, or sucrase;
- or (b) a microorganism expressing the enzymes, or a culture of the microorganism.
3 more elements
Dependencies are inferred from the claim text.