Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation

A composition of at least 1 mL in volume comprising ex-vivo expanded adipose tissue-derived stem cells (ASCs) mixed with harvested fat tissue at a ratio of at least 20×106ASCs/mL fat, wherein said ex-vivo expanded adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (a-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 10-20% pooled Human Platelet Lysate (pHPL) for at least 3 to 7 days, wherein said ex-vivo expanded adipose tissue-derived stem cells (ASCs) display a homogenous morphology, are adherent to a plastic culture surface, and are negative for the hematopoietic surface marker CD34.