Imaging-guided creating and monitoring of retinal vascular occlusive disease

A method comprising:

• acquiring, using an integrated optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) apparatus, baseline OCT and OCT angiography (OCTA) volumes of a subject;
• selecting a target blood vessel using the baseline OCT and OCT angiography (OCTA) volumes of the subject;
• injecting a photosensitive dye into the target blood vessel;
• visualizing the photosensitive dye in the target blood vessel using fluorescence angiograms obtained via an angiography protocol of a SLO subsystem of the apparatus;
• controlling, using the integrated OCT and SLO apparatus, a laser to form a vascular occlusion at a location on the target blood vessel using an actinic protocol of the SLO subsystem;
• wherein a power of the laser is higher for the actinic protocol than for the angiography protocol of the SLO subsystem;
• acquiring, using the integrated OCT and SLO apparatus, one or more subsequent OCT and OCTA volumes of the subject after the vascular occlusion;
• processing the OCT and OCTA volumes and feedback from the integrated OCT and SLO apparatus to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion.

At least one computer-readable storage medium including instructions that, when executed, cause at least one processor to at least:

• generate a location on a target vessel to control a laser in a scanning laser ophthalmoscope (SLO) to form a vascular occlusion at the location;
• wherein the vascular occlusion is formed using an actinic protocol of the SLO;
• process first data acquired from optical coherence tomography (OCT) and OCT angiography (OCTA) volumes including the target vessel obtained before the vascular occlusion in contrast with second data acquired from OCT and OCTA volumes including the target vessel obtained after the vascular occlusion to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion;
• wherein the subject is to be injected with a photosensitive dye before the vascular occlusion is formed;
• and wherein the dye is visualized using fluorescence angiograms obtained using an angiography protocol of the SLO;
• wherein a power of the laser is higher for the actinic protocol than for the angiography protocol;
• and
• generate a model of a subject including the target vessel based on the first data, the second data, and the change in the three-dimensional vasculature.