Tomato plants having fruit with yellow and red segments
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsJoseph Hirschberg, Dani Zamir, Yaacov Micha Brog, Itay Zemach, Orly Dery
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Abstract
Provided are tomato plants having fruit with red and yellow segments that appear across the fruit from the internal seed area to the most external layer of the epidermis. The present invention discloses that this phenotype, designated Arlecchino, is linked to insertion mutation within the Phytoene synthase 1 (Psy1) gene.
Claims13
A tomato cultivar producing fruit having an Arlecchino phenotype of yellow-red segments spanning from the placenta and/or locules across the fruit pericarp to the epidermis, wherein
- cells of the yellow segments are homozygous for an allele of rarl, the rarl allele is Phytoene synthase 1 (Psy1) allele comprising an insertion within an intron of the allele, wherein the insertion comprises a transposon within intron 8 of the allele, comprising a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:3, and flanked at the 3′ end by a nucleic acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2, and wherein the insertion results in a non-functional splice variant of Psy1;
- and
- cells of the red segments comprise:
- a. at least one wild type Psy1 allele;
- b. at least one Psy1 allele comprising transposon excision footprint, the transposon excision footprint comprising at least one nucleotide deletion within the nucleic acid sequence set forth in SEQ ID NO:7;
- or
- c. a combination of a and b.
A method for producing a tomato plant producing fruit having an Arlecchino phenotype of yellow-red segments spanning from the placenta and/or locules across the fruit pericarp to the epidermis, the method comprising:
- a. introducing into a tomato plant producing red fruit or a part thereof a genetic element comprising an rarl allele of Phytoene synthase 1(Psy1), wherein the rarl allele comprises an insertion within an intron of the allele, wherein the insertion comprises a transposon within intron 8 of the allele, comprising a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:3 and flanked at the 3′ end by a nucleic acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2, and wherein the insertion results in a non-functional splice variant of Psy1:
- and
- b. selfing the tomato plant comprising the rarl allele,
- thereby producing a fruit having the Arlecchino phenotype.
Dependencies are inferred from the claim text.