Functionalized gel beads
Assignee10x Genomics, Inc.
InventorsZachary Bent, Elliott Meer, Daniel Riordan, Paul Ryvkin, Niranjan Srinivas, Jessica Terry
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Abstract
The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.
Claims43
A method for generating nucleic acid barcode molecules, comprising:
- (a) providing a plurality of nucleic acid molecules coupled to a plurality of supports;
- and
- (b) coupling one or more additional nucleic acid molecules to nucleic acid molecules of said plurality of nucleic acid molecules coupled to said plurality of supports, thereby assembling a plurality of nucleic acid barcode molecules comprising barcode sequences coupled to said plurality of supports,
- wherein said plurality of nucleic acid barcode molecules comprises (i) a first set of nucleic acid barcode molecules coupled to a support of said plurality of supports and (ii) a second set of nucleic acid barcode molecules coupled to said support, wherein a first nucleic acid barcode molecule of said first set of nucleic acid barcode molecules comprises a first nucleic acid capture sequence and a second nucleic acid barcode molecule of said second set of nucleic acid barcode molecules comprises a second nucleic acid capture sequence, wherein said first nucleic acid capture sequence and said second nucleic acid capture sequence are independently selected from SEQ ID NO:1 through SEQ ID NO:163.
Dependencies are inferred from the claim text.