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US 10,745,725 B2Granted
Method for producing l-amino acid by increasing fructose uptake
AssigneeAjinomoto Co., Inc.
InventorsKosuke Yokota, Kazuyuki Hayashi
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Abstract
A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid, which has been modified so that the activity of a non-PTS fructose-uptake carrier and the activity of fructokinase are both increased, in a medium containing fructose, and collecting the L-amino acid from the medium or cells of the bacterium.
Claims18
A method for producing an L-amino acid, the method comprising:
- A) culturing a bacterium that is able to produce an L-amino acid in a medium containing fructose so that an L-amino acid is produced and accumulates in the medium and/or the bacterium;
- and
- B) collecting the L-amino acid from the medium and/or the bacterium,
- wherein the L-amino acid is selected from the group consisting of L-glutamic acid, L-glutamine, L-proline, L-arginine, L-citrulline, and L-ornithine,
- wherein the bacterium has been modified so that the activity of both a non-PTS fructose-uptake carrier and a fructokinase are increased as compared with a non-modified bacterium by increasing the expression of a gene encoding the non-PTS fructose-uptake carrier and a gene encoding the fructokinase, respectively,
- wherein the non-PTS fructose-uptake carrier is a protein selected from the group consisting of:
- (1a) a protein comprising the amino acid sequence of SEQ ID NO:
- 27 or 29;
- (1b) a protein comprising the amino acid sequence of SEQ ID NO:
- 27 or 29, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein comprises non-PTS fructose-uptake activity;
- and
- (1c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 27 or 29, and wherein said protein comprises non-PTS fructose-uptake activity, and
- wherein the fructokinase is a protein selected from the group consisting of:
- (2a) a protein comprising the amino acid sequence of SEQ ID NO:
- 31, 33, or 35;
- (2b) a protein comprising the amino acid sequence of SEQ ID NO:
- 31, 33, or 35, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein comprises fructokinase activity;
- and
- (2c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 31, 33, or 35, and wherein said protein comprises fructokinase activity.
18 more elements
Dependencies are inferred from the claim text.