Vectors and methods for targeted integration in loci comprising constitutively expressed genes
AssigneeMurdoch Childrens Research Institute
InventorsEd Stanley, Andrew Elefanty, David Elliott, Tatiana Labonne
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Abstract
The invention relates to a vector comprising: a 5′ nucleic acid that is homologous to a genomic sequence 5′ of a stop codon of a constitutively expressed gene; an exogenous nucleic acid; a 3′ nucleic acid that is homologous to a genomic sequence 3′ of the stop codon of the constitutively expressed gene; a translation interruption-reinitiation signal operably linked to the 5′ nucleic acid and the exogenous nucleic acid, wherein the translation interruption-reinitiation signal is capable of replacing the stop codon of the constitutively expressed gene.
Claims18
A vector for targeted integration of one or more exogenous nucleic acids into a constitutively expressed gene, the vector comprising:
- a 5′ nucleic acid that is homologous to a genomic sequence 5′ of a genomic stop codon of the constitutively expressed gene;
- an exogenous nucleic acid;
- a 3′ nucleic acid that is homologous to a genomic sequence 3′ of the genomic stop codon of the constitutively expressed gene;
- and
- a translation interruption-reinitiation signal operably linked to the 5′ nucleic acid and the exogenous nucleic acid,
- wherein the constitutively expressed gene comprises GAPDH and the translation interruption-reinitiation signal is capable of replacing the stop codon of the constitutively expressed gene.
Dependencies are inferred from the claim text.