Microfluidic device for in vitro 3D cell culture experimentation
AssigneeIntecrypt B.V.
InventorMikhail Alexandrovich Ponomarenko
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Abstract
A microfluidic device for in vitro 3D cell culture experimentation comprises a body in which is provided a cell culture chamber that is at least partly filled with a scaffolding substance for maintaining a cell culture. In the body a fluid path is provided that communicates with the cell culture chamber for directing a fluid stream along the scaffolding substance. The culture chamber above the scaffolding substance opens into an access port which is provided at an outer top surface of the body to provide direct access to the scaffolding substance. The scaffolding substance forms a fluid flow barrier which separates the fluid path from the access port.
Claims21
A microfluidic device for in vitro 3D cell culture experimentation comprising a body in which is provided:
- a cell culture chamber at least partly filled with a scaffolding substance for maintaining a cell culture;
- and
- a fluid path communicating with the cell culture chamber for directing a fluid stream along the scaffolding substance;
- wherein the culture chamber above the scaffolding substance opens into an access port provided at an outer top surface of the body for direct access to the scaffolding substance which forms a fluid flow barrier separating the fluid path from the access port, wherein the access port is provided directly above the cell culture chamber, and wherein the body comprises a restricting wall between the access port and the culture chamber, the restricting wall comprising at least one passage opening of which a cross sectional size is smaller than the cross section size of the culture chamber or the cross sectional size of the access port to restrict fluid communication between the access port and the culture chamber.
Dependencies are inferred from the claim text.