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US 10,744,477 B2Granted
Devices and methods for oligonucleic acid library synthesis
AssigneeTwist Bioscience Corporation
InventorsWilliam Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle, Eugene P. Marsh
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Abstract
Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.
Claims17
A device for synthesizing oligonucleotides, comprising:
- a solid substrate;
- a main channel, wherein the main channel extends vertically into the solid substrate from an opening on a top side of the solid substrate;
- and
- a plurality of microchannels, wherein each microchannel of the plurality of microchannels extends vertically from an opening on a bottom side of the solid substrate into the main channel, and wherein each microchannel of the plurality of microchannels comprises a fluid path having at least one turn of 45 degrees to 180 degrees in total, when viewed from a top view, and
- wherein the device comprises more than 20,000 of the microchannels in total.
2 more elements
Dependencies are inferred from the claim text.