Back to results
US 10,744,230 B2Granted
Biomimetic hybrid gel compositions and methods of use
AssigneesBioactive Regenerative Therapeutics, Inc., Regents of the University of Minnesota
InventorsJohn H. Brekke, Timothy O'Brien
Life of this patent
Estimating the term…
Abstract
Disclosed herein are dry blends of polyanionic and polycationic macromolecules, solvating fluids serving as cell suspension fluids, hybrid gel compositions, and methods for treatment of patients with endocrine disorders by transplantation with such compositions. Hybrid gel compositions that promote a microenvironment suitable for cell viability and growth while maintaining a sufficient structural integrity for three-dimensional cell culture are also disclosed.
Claims18
A method for forming a three-dimensional hybrid gel composition comprising:
- combining dextran sulfate and a solvating fluid to form a composition;
- adding at least one polycationic macromolecule to the composition to form the three-dimensional hybrid gel composition, which comprises a network of insoluble, polyelectrolytic complex structures having regions of unreacted polycationic macromolecules;
- and
- suspending in the solvating fluid cells selected from the group consisting of endocrine cells and pluripotent cells.
1 more element
Dependencies are inferred from the claim text.