Thiolated devitalized tissue particle implantable hydrogel
AssigneeThe Board of Regents of the University of Oklahoma
InventorsJakob M. Townsend, Michael S. Detamore
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Abstract
Methods and compositions for use in repairing and/or regenerating tissues are disclosed. A hydrogel precursor composition comprising functionalized tissue particles and functionalized hyaluronic acid that can be deposited into an implant site and converted into a crosslinked hydrogel network which retains the tissue particles at the implant site is disclosed. Also disclosed are methods of forming functionalized tissue particles and functionalized hyaluronic acid for use herein. Kits containing reagents for producing the hydrogel precursor composition are also disclosed, along with methods of producing the hydrogel precursor composition.
Claims46
An implantable hydrogel precursor composition, comprising:
- a devitalized tissue particle composition comprising thiolated devitalized tissue particles;
- a hyaluronic acid composition comprising functionalized hyaluronic acid molecules;
- and
- a photoinitiator for initiating covalent linkage of the functionalized hyaluronic acid molecules to the thiolated devitalized tissue particles;
- and
- wherein the devitalized tissue particle composition, the hyaluronic acid composition, and the photoinitiator form a hydrogel precursor composition when combined, and wherein the hydrogel precursor composition is capable of forming a crosslinked hydrogel when exposed to a crosslink-activating wavelength.
A kit for use in repair or regeneration of a damaged tissue, the kit comprising:
- one or more containers comprising:
- a devitalized tissue particle composition comprising thiolated devitalized tissue particles;
- a hyaluronic acid composition comprising functionalized hyaluronic acid molecules;
- and
- a photoinitiator for initiating covalent linkage of the functionalized hyaluronic acid molecules to the thiolated devitalized tissue particles;
- and
- wherein when combined, the devitalized tissue particle composition, the hyaluronic acid composition, and the photoinitiator form a hydrogel precursor composition, and wherein the hydrogel precursor composition is capable of forming a crosslinked hydrogel when exposed to a crosslink-activating wavelength.
A method of forming a hydrogel implant in an implant site in vivo, the method comprising the steps of:
- forming a hydrogel precursor composition by combining:
- (1) a devitalized tissue particle composition comprising thiolated devitalized tissue particles;
- (2) a hyaluronic acid composition comprising functionalized hyaluronic acid molecules;
- and
- (3) a photoinitiator for initiating covalent linkage of the functionalized hyaluronic acid molecules to the thiolated devitalized tissue particles to cause crosslinking of the functionalized hyaluronic acid molecules;
- depositing a quantity of the hydrogel precursor composition into the implant site;
- and
- irradiating the deposited hydrogel precursor composition with a crosslink-activating wavelength causing crosslinking of the functionalized hyaluronic acid molecules within the deposited hydrogel precursor composition to form a crosslinked hydrogel implant in the implant site.
A hydrogel implant, comprising:
- a hydrogel precursor composition that has been implanted into an implant site in vivo and exposed to a crosslink-activating wavelength, thereby forming a crosslinked hydrogel, the hydrogel precursor composition comprising thiolated devitalized tissue particles, functionalized hyaluronic acid molecules, and a photoinitiator, wherein the functionalized hyaluronic acid molecules are crosslinked via covalent linkages to the thiolated devitalized tissue particles upon exposure of the hydrogel precursor composition to the crosslink-activating wavelength.
Dependencies are inferred from the claim text.