High-throughput method for detecting tooth stain prevention and removal
AssigneeJohnson & Johnson Consumer Inc.
InventorsMichael C. Giano, Daniel Queiroz, Susan Knox
Life of this patent
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Abstract
Provided are methods of detecting tooth stain prevention and/or removal comprising the steps of providing pellicle-coated hydroxyapatite particles, contacting the particles with a first solution and then a second solution, wherein the first solution is either a test solution or a stain solution and the second solution is the other of said test solution or stain solution, separating from the resulting particles a liquid comprising stain introduced in the contacting step b), and measuring the UV-Vis absorbance of the separated liquid to determine the amount of stain in the liquid.
Claims9
A high-throughput method of detecting tooth stain prevention or removal comprising the steps of:
- a. providing pellicle-coated hydroxyapatite particles having a particle size of greater than about 3 micron to about 200 micron in a multi-well filter container,
- b. contacting said particles with a first solution and then a second solution in the multi-well filter container, wherein said first solution is either a test solution or a stain solution and the second solution is the other of said test solution or stain solution,
- c. separating from said particles from said solution comprising stain introduced in the contacting step b), and
- d. measuring the UV-Vis absorbance of the solution in a multimode plate reader separated in step c) to determine the amount of stain in such solution wherein the multi-well filter container and multimode plate reader are used to provide higher throughput.
Dependencies are inferred from the claim text.