Stem cell material and method of manufacturing
AssigneeT-Helper Cell Technologies, LLC
InventorsAnatoliy Sokolov, Antonina Ivanovna Kolesnikova, Andrei Igorevich Dovgii
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Abstract
Aspects of the invention relate to a novel mesenchymal stem cell line (hb-MSC), a culture medium conditioned by the hb-MSC line, and various hb-MSC compositions. The hb-MSC composition may include a plurality of hb-MSCs, an hb-MSC conditioned medium, or a combination thereof. The hb-MSC composition may also include an appropriate carrier. Also described are methods of use for the hb-MSC cells, the conditioned medium and compositions thereof.
Claims31
A composition comprising:
- (a) an artificial culture medium conditioned by a population of non-fetal mesenchymal stem cells seeded at 0.7×106 cells per 75 cm2 surface area, and
- (b) some or all of said population of non-fetal mesenchymal stem cells,
- wherein the conditioned medium contains at least 760 pg/ml of vascular endothelial growth factor (VEGF) and at least 150 pg/ml of growth-regulated oncogene/keratinocyte chemoattractant (GRO/KC).
A composition comprising, a mixture of factors comprising VEGF, GRO/KC, TGF-β1, and TGF-β2, wherein VEGF is present at a concentration of at least 760 pg/ml and GRO/KC is present at a concentration of at least 150 pg/ml, and a carrier.
A composition comprising:
- (a) an artificial culture medium conditioned by a population of non-fetal mesenchymal stem cells, and
- (b) some or all of the population of said non-fetal mesenchymal stem cells,
- wherein the conditioned medium contains transforming growth factor-β1 (TGF-β1), transforming growth factor-β2 (TGF-β2), at least 760 pg/ml of vascular endothelial growth factor (VEGF) and at least 150 pg/ml of growth-regulated oncogene/keratinocyte chemoattractant (GRO/KC).
Dependencies are inferred from the claim text.