US 10,744,159 B2 · Method for evaluating mesenchymal stem cell activity, method for culturing mesenchymal stem cells, method for producing therapeutic agent for liver dysfunction, and therapeutic agent for liver dysfunction

Abstract

A method for evaluating the activity level of mesenchymal stem cells, and a method for culturing mesenchymal stem cells using the evaluation method in the field of culturing mesenchymal stem cells for regenerative medicine, and further, a method for producing a therapeutic agent for liver dysfunction and a therapeutic agent for liver dysfunction. This method for evaluating mesenchymal stem cell activity has an assay step for assaying the amount of adenylate kinase 4 (AK4) in the mesenchymal stem cells; and a determination step for determining the activity level of the mesenchymal stem cells from the assayed amount of adenylate kinase 4.

Claims7

Dependent claims

A method of culturing mesenchymal stem cells comprising the steps of:

isolating mesenchymal stem cells from a subject fluid containing mesenchymal stem cells;
culturing the isolated mesenchymal stem cells;
sampling a part of the cultured mesenchymal stem cells;
assaying the amount of adenylate kinase 4 in the sampled mesenchymal stem cells;
and
subculturing the assayed sampled mesenchymal stem cells if the assayed amount of adenylate kinase 4 is equal to or higher than a threshold α which is greater than the assayed expression of adenylate kinase 4 when the mesenchymal stem cells are cultured under conditions at 20% oxygen.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/512,390Filed 19 Aug 2015

Priority

JP 2014-18937817 Sep 2014

Published

US 2017/0246211 A131 Aug 2017

Granted

18 Aug 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

A61K 35/28
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 35/12
Medicinal preparations containing materials or reaction products thereof with undetermined constitution: materials from mammals; Compositions…
A61K 45/00
Medicinal preparations containing active ingredients not provided for in groups a61k31/00 -
A61P 1/16
Drugs for disorders of the alimentary tract or the digestive system: for liver or gallbladder disorders, e.g. hepatoprotective agents,…

Method for evaluating mesenchymal stem cell activity, method for culturing mesenchymal stem cells, method for producing therapeutic agent for liver dysfunction, and therapeutic agent for liver dysfunction

Life of this patent

Method for evaluating mesenchymal stem cell activity, method for culturing mesenchymal stem cells, method for producing therapeutic agent for liver dysfunction, and therapeutic agent for liver dysfunction

Life of this patent

Abstract

Claims7