Method for extracting high-purity mogroside V from Siraitia grosvenorii
AssigneeJiangxi Haifu Bioengineering Co., Ltd.
InventorsWeian Long, Huaxue Huang
Life of this patent
Estimating the term…
Abstract
The present application relates to a technique for extracting mogroside V. Provided is a method for extracting high-purity mogroside V from Siraitia grosvenorii. The specific steps comprise: pre-treatment of a raw material, extraction, centrifugation, enzymolysis, ultrafiltration, nanofiltration, decolorization, concentration, microwave drying, and pulverization. The invention utilizes a membrane-based technique for separation and purification, and only uses pure water as a solvent to eliminate usage of an organic solvent. The method can be easily performed, has a simple process, and provides a safe, environment-friendly, high quality, and low-cost product. The method can be used to realize continuous large-scale industrial production.
Claims10
A preparation method for extracting high-purity mogroside V from Luo Han Guo, characterized in that, the method comprises the following steps:
- (1) pre-treatment of raw material:
- crushing a raw material by using a crusher in accordance with the standard of one fresh fruit being broken into 6 to 10 pieces;
- (2) extraction:
- by means of continuous countercurrent extraction method, subjecting the raw material obtained in step (1) to hot water continuous countercurrent extraction to extract mogroside V;
- and passing the extracted liquid through a 200 to 400-mesh stainless steel sieve to obtain a filtrate;
- (3) centrifugation:
- subjecting the filtrate obtained in step (2) to centrifugal filtration to obtain a centrifugate;
- (4) enzymolysis:
- cooling down the centrifugate obtained in step (3), subsequently delivering it to an enzymolysis tank, and adding an enzyme reagent therein to perform enzymolysis;
- (5) ultrafiltration:
- passing the enzymatic centrifugate obtained in step (4) through an ultrafiltration membrane to concentrate to small-volume solution, adding water therein to perform dialysis until the conductivity of the solution meeting the requirement, and obtaining a filtrate;
- (6) nanofiltration:
- using a nanofiltration membrane to separate and purify the filtrate obtained in step (5), concentrating it to small-volume solution, adding water therein to perform dialysis until the conductivity of the solution meeting the requirement, and obtaining an intercepted liquid;
- (7) decolorization:
- using a decolorizing resin column to decolorize the intercepted liquid obtained in step (6), collecting the effluent liquid flowing through the decolorizing resin column after decolorization, and obtaining a decolorized liquid;
- (8) concentration:
- subjecting the decolorized liquid obtained in step (7) to vacuum concentration with a pressure controlled in the range of −0.1˜0.06 MPa and a temperature controlled in the range of 40˜50° C. until solid content is in the range of 40˜60%, and obtaining a concentrated liquid;
- and
- (9) microwave drying and pulverization:
- subjecting the concentrated liquid obtained in step (9) to microwave drying, pulverizing and sieving, and finally packing.
Dependencies are inferred from the claim text.