Back to results
US 10,743,526 B2Granted
Recombinant constructs and transgenic fluorescent ornamental fish therefrom
AssigneeGloFish, LLC
InventorsAlan Blake, Richard Crockett, Jeffrey Essner, Perry Hackett, Aidas Nasevicius
Life of this patent
Estimating the term…
Abstract
The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
Claims13
A transgenic fluorescent fish comprising in its genome:
- (a) a first transgene encoding a first fluorescent protein, wherein the first transgene is under the control of a muscle specific promoter, (b) a second transgene encoding a second fluorescent protein, wherein the second transgene is under the control of a ubiquitous fish promoter, and (c) a third transgene encoding a third fluorescent protein, wherein the third transgene is under the control of a ubiquitous fish promoter;
- wherein the first and second transgene encode different fluorescent proteins, and wherein the fluorescent fish visibly expresses a fluorescent color from each of the first and second transgene, and wherein the transgenic fluorescent fish exhibits a strong visible fluorescence over 75% to 100% of the body of said fish, optionally excluding fins and eyes, wherein at least one of the first and second transgene comprises a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5.
A transgenic fluorescent fish comprising in its genome:
- (a) a first transgene encoding a first fluorescent protein, wherein the first transgene is under the control of a muscle specific promoter, (b) a second transgene encoding a second fluorescent protein, wherein the second transgene is under the control of a muscle specific promoter, and (c) a third transgene encoding a third fluorescent protein, wherein the third transgene is under the control of a muscle specific promoter;
- wherein the first and second transgene encode different fluorescent proteins, and wherein the fluorescent fish visibly expresses a fluorescent color different from the color encoded by the first and second transgene, and wherein the transgenic fluorescent fish exhibits a strong visible fluorescence over 75% to 100% of the body of said fish, optionally excluding fins and eyes, wherein at least one of the first and second transgene comprises a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3 and SEQ ID NO:4.
Dependencies are inferred from the claim text.