Production of itaconic acid and related molecules from aromatic compounds
AssigneesUT-Battelle, LLC, Alliance for Sustainable Energy, LLC
InventorsJoshua R. Elmore, Jay Huenemann, Davinia Salvachua, Gregg T. Beckham, Adam M. Guss
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Abstract
This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.
Claims52
A genetically-modified bacterium from the genus Pseudomonas comprising an exogenous nucleic acid encoding a cis-aconitate decarboxylase having a protein sequence with at least 95% identity to SEQ ID NO:
- 108, wherein the endogenous genes that encode polyhydroxyalkanoate (PHA) synthases are mutated to be inactivated in the bacterium.
A genetically-modified bacterium from the genus Pseudomonas comprising an exogenous nucleic acid encoding an aconitate isomerase having a protein sequence with at least 95% identity to SEQ ID NO:
- 110, wherein the endogenous genes that encode polyhydroxyalkanoate (PHA) synthases are mutated to be inactivated in the bacterium.
Dependencies are inferred from the claim text.