Transformant for producing 4-hydroxybenzoic acid or salt thereof
AssigneeGreen Chemicals Co., Ltd.
InventorsMasayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
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Abstract
The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.
Claims7
A transformant obtained by introducing into a coryneform bacterium host cell a DNA which encodes a mutant chorismate-pyruvate lyase, wherein the mutant chorismate-pyruvate lyase is a variant of the polypeptide of SEQ ID NO:
- 1, wherein said variant has at least 90% sequence identity with the polypeptide of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8 or 9, wherein said variant has chorismate-pyruvate lyase activity, and wherein said variant comprises an alanine, cysteine, threonine, serine, or asparagine at the position corresponding to position 80 of the polypeptide of SEQ ID NO:
- 1.
A Corynebacterium glutamicum transformant, wherein said transformant is Corynebacterium glutamicum HBA-47 deposited at NITE under (Accession Number BP-01849.
Dependencies are inferred from the claim text.