Conjugation of Staphylococcus aureus type 5 and type 8 capsular polysaccharides
AssigneeGlaxosmithkline Biologicals SA
InventorsFrancesco Berti, Paolo Costantino, Maria Rosaria Romano
Life of this patent
Estimating the term…
Abstract
The invention provides a process for preparing a conjugate of a S. aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.
Claims10
A process for preparing a conjugate of a fragment of an S. aureus type 5 capsular polysaccharide and a carrier molecule, comprising the steps of:
- (a) depolymerizing a full-length type 5 capsular polysaccharide to prepare a fragment of the type 5 capsular polysaccharide having a P3-D-FucNAc-(1→moiety at its non-reducing terminus;
- (b) oxidising the fragment of the type 5 capsular polysaccharide to introduce an aldehyde group into at least one saccharide residue in the fragment to prepare a fragment having at least one oxidised saccharide residue;
- and
- (c) coupling the fragment having the at least one oxidised saccharide residue to the carrier molecule via the aldehyde group,
- thereby preparing the conjugate.
Dependencies are inferred from the claim text.