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US 10,731,202 B2Granted
Chromatin activity precipitation method and system
AssigneeBattelle Memorial Institute
InventorsStephen R. Lindemann, Aaron T. Wright
Life of this patent
Estimating the term…
Abstract
Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins.
Claims14
A method of identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules comprising:
- a. providing a reactive probe that mimics small molecule cofactors;
- and
- b. irreversibly binding a target macromolecule to the probe to selectively pull down or precipitate probe-bound macromolecules that comprise target nucleic acids, wherein both captured and uncaptured macromolecule fragments are sequenced in parallel to detect which fragments are enriched or depleted.
Dependencies are inferred from the claim text.